+Open data
-Basic information
Entry | Database: PDB / ID: 1gxv | ||||||
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Title | Solution structure of lysozyme at low and high pressure | ||||||
Components | LYSOZYME C | ||||||
Keywords | HYDROLASE / SACCHARIDE DEGRADATION / GLYCOSIDASE / BACTERIOLYTIC ENZYME / ALLERGEN / EGG-WHITE | ||||||
Function / homology | Function and homology information Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium ...Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | GALLUS GALLUS (chicken) | ||||||
Method | SOLUTION NMR / CHEMICAL SHIFT REFINEMENT | ||||||
Model type details | MINIMIZED AVERAGE | ||||||
Authors | Refaee, M. / Akasaka, K. / Williamson, M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Pressure-Dependent Changes in the Solution Structure of Hen Egg-White Lysozyme Authors: Refaee, M. / Tezuka, T. / Akasaka, K. / Williamson, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gxv.cif.gz | 52.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gxv.ent.gz | 41.4 KB | Display | PDB format |
PDBx/mmJSON format | 1gxv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gx/1gxv ftp://data.pdbj.org/pub/pdb/validation_reports/gx/1gxv | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) GALLUS GALLUS (chicken) / Organ: EGG-WHITE / References: UniProt: P00698, lysozyme |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: NOESY |
NMR details | Text: MEAN STRUCTURE. THE STRUCTURE WAS DETERMINED BY ENERGY REFINEMENT OF THE 2LYM CRYSTAL STRUCTURE, FOR COMPARISON WITH THE HIGH-PRESSURE STRUCTURE |
-Sample preparation
Sample conditions | Ionic strength: 0.1 / pH: 2.0 / Pressure: 1 atm / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 750 MHz |
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-Processing
NMR software |
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Refinement | Method: CHEMICAL SHIFT REFINEMENT / Software ordinal: 1 Details: REFINEMENT USED FULL FORCEFIELD, RESTRAINING 1H CHEMICAL SHIFTS TO THOSE CALCULATED | |||||||||
NMR ensemble | Conformer selection criteria: ENERGY MINIMISED / Conformers calculated total number: 50 / Conformers submitted total number: 1 |