[English] 日本語
![](img/lk-miru.gif)
- PDB-1ja6: BINDING OF N-ACETYLGLUCOSAMINE TO CHICKEN EGG LYSOZYME: A POWDER ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1ja6 | ||||||
---|---|---|---|---|---|---|---|
Title | BINDING OF N-ACETYLGLUCOSAMINE TO CHICKEN EGG LYSOZYME: A POWDER DIFFRACTION STUDY | ||||||
![]() | LYSOZYME | ||||||
![]() | HYDROLASE / POWDER DIFFRACTION / RIETVELD REFINEMENT / LYSOZYME | ||||||
Function / homology | ![]() Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium ...Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | POWDER DIFFRACTION / ![]() ![]() | ||||||
![]() | Von Dreele, R.B. | ||||||
![]() | ![]() Title: Binding of N-acetylglucosamine to chicken egg lysozyme: a powder diffraction study. Authors: Von Dreele, R.B. #1: ![]() Title: Combined Rietveld and Stereochemical Restraint Refinement of a Protein Crystal Structure Authors: Von Dreele, R.B. #2: ![]() Title: The First Protein Crystal Structure Determined from Resolution X-Ray Powder Diffraction Data: A Variant of the T3R3 Human Insulin Zinc Complex Produced by Grinding Authors: Von Dreele, R.B. / Stephens, P.W. / Blessing, R.H. / Smith, G.D. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 32.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 19.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1ja2C ![]() 1ja4C ![]() 1ja7C ![]() 1rfpS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: POWDER DIFFRACTION / Number of used crystals: 1 |
---|
-
Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.77 % |
---|---|
Crystal grow | Temperature: 296 K / Method: precipitation / pH: 5.2 Details: rapid precipitation from 0.5M NACL IN PH 6.0 0.05M NA2HPO4/KH2PO4 BUFFER, at 296K, pH 5.2 |
Crystal grow | *PLUS Method: unknown / Details: Von Dreele, R.B., (2000) Acta Cryst., D56, 1549. |
Components of the solutions | *PLUS Conc.: 1.0 M / Chemical formula: NaCl |
-Data collection
Diffraction | Mean temperature: 296 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: SCINTILLATOR / Date: Oct 22, 2000 / Details: 2MM X 8MM BEAM |
Radiation | Monochromator: DOUBLE SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.69997 Å / Relative weight: 1 |
Reflection | Resolution: 2.96→40.11 Å / Num. all: 2824 / Num. obs: 2824 / % possible obs: 100 % |
-
Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1RFP Resolution: 2.96→40.11 Å / Num. reflection all: 2824 / Num. reflection obs: 2824 / Isotropic thermal model: Overall fixed Details: Used band diagonal matrix least squares with 300 parameter bandwidth | ||||||||||||
Displacement parameters | Biso mean: 23.69 Å2 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.96→40.11 Å
| ||||||||||||
Refine LS restraints |
|