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- PDB-2iff: STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATI... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2iff | ||||||
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Title | STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION | ||||||
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![]() | IMMUNOGLOBULIN/HYDROLASE(O-GLYCOSYL) / IMMUNOGLOBULIN-HYDROLASE(O-GLYCOSYL) complex | ||||||
Function / homology | ![]() Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium ...Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Chacko, S. / Davies, D.R. | ||||||
![]() | ![]() Title: Structure of an antibody-lysozyme complex unexpected effect of conservative mutation. Authors: Chacko, S. / Silverton, E. / Kam-Morgan, L. / Smith-Gill, S. / Cohen, G. / Davies, D. #1: ![]() Title: Three-Dimensional Structure of an Antibody-Antigen Complex Authors: Sheriff, S. / Silverton, E.W. / Padlan, E.A. / Cohen, G.H. / Smith-Gill, S.J. / Finzel, B.C. / Davies, D.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 119.9 KB | Display | ![]() |
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PDB format | ![]() | 92.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.2 KB | Display | ![]() |
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Full document | ![]() | 422.3 KB | Display | |
Data in XML | ![]() | 14.3 KB | Display | |
Data in CIF | ![]() | 21.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO L 8 / 2: CIS PROLINE - PRO L 139 / 3: CIS PROLINE - PRO H 150 / 4: CIS PROLINE - PRO H 152 / 5: CIS PROLINE - PRO H 192 |
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Components
#1: Antibody | Mass: 23326.729 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Antibody | Mass: 22975.445 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Protein | Mass: 14303.146 Da / Num. of mol.: 1 / Mutation: R68K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#4: Water | ChemComp-HOH / |
Sequence details | THE LIGHT CHAIN OF THE FAB HAS CHAIN INDICATOR L. THE HEAVY CHAIN OF THE FAB HAS CHAIN INDICATOR H. ...THE LIGHT CHAIN OF THE FAB HAS CHAIN INDICATOR L. THE HEAVY CHAIN OF THE FAB HAS CHAIN INDICATOR H. THE LYSOZYME HAS CHAIN INDICATOR Y. THE NUMBERING SYSTEM USED IN THIS ENTRY IS SEQUENTIAL |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 52.97 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.2 / Method: unknown | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 9999 Å / Num. obs: 12909 / % possible obs: 66.4 % / Num. measured all: 37251 / Rmerge(I) obs: 0.076 |
Reflection shell | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 2.76 Å / % possible obs: 22.3 % / Num. unique obs: 717 / Num. measured obs: 1140 / Mean I/σ(I) obs: 3.82 |
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Processing
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Refinement | Resolution: 2.65→10 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.65→10 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.183 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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