+Open data
-Basic information
Entry | Database: PDB / ID: 1uco | ||||||
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Title | HEN EGG-WHITE LYSOZYME, LOW HUMIDITY FORM | ||||||
Components | LYSOZYME | ||||||
Keywords | HYDROLASE (O-GLYCOSYL) / ENZYME-MONOCLINIC FORM / HYDROLASE / O-GLYCOSYL | ||||||
Function / homology | Function and homology information Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium ...Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Nagendra, H.G. / Sudarsanakumar, C. / Vijayan, M. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1996 Title: An X-ray analysis of native monoclinic lysozyme. A case study on the reliability of refined protein structures and a comparison with the low-humidity form in relation to mobility and enzyme action. Authors: Nagendra, H.G. / Sudarsanakumar, C. / Vijayan, M. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1993 Title: Protein Hydration and Water Structure: X-Ray Analysis of a Closely Packed Protein Crystal with Very Low Solvent Content Authors: Madhusudan / Kodandapani, R. / Vijayan, M. #2: Journal: J.Appl.Crystallogr. / Year: 1993 Title: Comparison of Radiation Induced Decay and Structure Refinement from X-Ray Data Collected from Lysozyme Crystals at Low and Ambient Temperatures Authors: Young, A.C.M. / Dewan, J.C. / Nave, C. / Tilton, R.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uco.cif.gz | 67.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uco.ent.gz | 50.2 KB | Display | PDB format |
PDBx/mmJSON format | 1uco.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1uco_validation.pdf.gz | 431.2 KB | Display | wwPDB validaton report |
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Full document | 1uco_full_validation.pdf.gz | 446.1 KB | Display | |
Data in XML | 1uco_validation.xml.gz | 16.9 KB | Display | |
Data in CIF | 1uco_validation.cif.gz | 24 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uc/1uco ftp://data.pdbj.org/pub/pdb/validation_reports/uc/1uco | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: CRYSTAL STRUCTURE OF NATIVE MONOCLINIC LYSOZYME / Source: (natural) Gallus gallus (chicken) / Cell: EGG / Cellular location: WHITE / References: UniProt: P00698, lysozyme #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.85 Å3/Da / Density % sol: 32.4 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 4.6 / Method: unknown | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Jan 9, 1991 |
Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 12993 / % possible obs: 86.6 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.0538 |
Reflection | *PLUS Highest resolution: 2 Å / Rmerge(I) obs: 0.053 |
-Processing
Software |
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Refinement | Resolution: 2→10 Å / σ(F): 4 /
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Displacement parameters | Biso mean: 14.53 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.17 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.169 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |