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- PDB-2yo3: Salmonella enterica SadA 1185-1386 fused to GCN4 adaptors (SadAK14) -
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Open data
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Basic information
Entry | Database: PDB / ID: 2yo3 | ||||||
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Title | Salmonella enterica SadA 1185-1386 fused to GCN4 adaptors (SadAK14) | ||||||
![]() | GENERAL CONTROL PROTEIN GCN4, PUTATIVE INNER MEMBRANE PROTEIN, GENERAL CONTROL PROTEIN GCN4 | ||||||
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Function / homology | ![]() protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hartmann, M.D. / Hernandez Alvarez, B. / Lupas, A.N. | ||||||
![]() | ![]() Title: Complete Fiber Structures of Complex Trimeric Autotransporter Adhesins Conserved in Enterobacteria. Authors: Hartmann, M.D. / Grin, I. / Dunin-Horkawicz, S. / Deiss, S. / Linke, D. / Lupas, A.N. / Hernandez Alvarez, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 319.7 KB | Display | ![]() |
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PDB format | ![]() | 262.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2ynyC ![]() 2ynzC ![]() 2yo0C ![]() 2yo1C ![]() 2yo2C ![]() 1gcmS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / End auth comp-ID: LYS / End label comp-ID: LYS
NCS ensembles :
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Components
#1: Protein | Mass: 28931.012 Da / Num. of mol.: 3 Fragment: GCN4 ADAPTOR RESIDUES 250-278, ADHESIN RESIDUES 1185-1386, GCN4 ADAPTOR RESIDUES 250-278 Mutation: YES Source method: isolated from a genetically manipulated source Details: N- AND C-TERMINAL IN-REGISTER FUSION TO GCN4 ADAPTORS Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() Production host: ![]() ![]() ![]() #2: Chemical | ChemComp-CL / | ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50 % / Description: NONE |
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Crystal grow![]() | Details: 12% (W/V) PEG 8000, 100 MM MAGNESIUM ACETATE, 100 MM TRIS PH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 29, 2008 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→37.3 Å / Num. obs: 59695 / % possible obs: 98.3 % / Redundancy: 3.91 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 2→2.12 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.61 / Mean I/σ(I) obs: 2.32 / % possible all: 96.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1GCM Resolution: 2→37.31 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.919 / SU B: 10.465 / SU ML: 0.127 / Cross valid method: THROUGHOUT / ESU R: 0.181 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.737 Å2
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Refinement step | Cycle: LAST / Resolution: 2→37.31 Å
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Refine LS restraints |
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