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- PDB-1fav: THE STRUCTURE OF AN HIV-1 SPECIFIC CELL ENTRY INHIBITOR IN COMPLE... -
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Basic information
Entry | Database: PDB / ID: 1fav | |||||||||
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Title | THE STRUCTURE OF AN HIV-1 SPECIFIC CELL ENTRY INHIBITOR IN COMPLEX WITH THE HIV-1 GP41 TRIMERIC CORE | |||||||||
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![]() | VIRAL PROTEIN / HIV-1 / gp41 / inhibitor | |||||||||
Function / homology | ![]() nitrogen catabolite activation of transcription from RNA polymerase II promoter / FCERI mediated MAPK activation / Synthesis and processing of ENV and VPU / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / symbiont-mediated evasion of host immune response / mediator complex binding ...nitrogen catabolite activation of transcription from RNA polymerase II promoter / FCERI mediated MAPK activation / Synthesis and processing of ENV and VPU / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / symbiont-mediated evasion of host immune response / mediator complex binding / Alpha-defensins / Oxidative Stress Induced Senescence / Dectin-2 family / TFIID-class transcription factor complex binding / amino acid biosynthetic process / positive regulation of establishment of T cell polarity / positive regulation of RNA polymerase II transcription preinitiation complex assembly / positive regulation of transcription initiation by RNA polymerase II / Binding and entry of HIV virion / cellular response to nutrient levels / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / cellular response to amino acid starvation / host cell endosome membrane / actin filament organization / Assembly Of The HIV Virion / Budding and maturation of HIV virion / RNA polymerase II transcription regulator complex / DNA-binding transcription activator activity, RNA polymerase II-specific / clathrin-dependent endocytosis of virus by host cell / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / viral protein processing / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / : / viral envelope / chromatin binding / apoptotic process / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Zhou, G. / Ferrer, M. / Chopra, R. / Strassmaier, T. / Weissenhorn, W. / Skehel, J.J. / Oprian, D. / Schreiber, S.L. / Harrison, S.C. / Wiley, D.C. | |||||||||
![]() | ![]() Title: The structure of an HIV-1 specific cell entry inhibitor in complex with the HIV-1 gp41 trimeric core. Authors: Zhou, G. / Ferrer, M. / Chopra, R. / Kapoor, T.M. / Strassmaier, T. / Weissenhorn, W. / Skehel, J.J. / Oprian, D. / Schreiber, S.L. / Harrison, S.C. / Wiley, D.C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 35.4 KB | Display | ![]() |
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PDB format | ![]() | 24.8 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 436.6 KB | Display | ![]() |
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Full document | ![]() | 438 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 8.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9268.784 Da / Num. of mol.: 1 / Mutation: L2I, V6I, L9I, N13I, L16I, V20I, L23I, V27I Source method: isolated from a genetically manipulated source Details: CHIMERA CONSISTS OF A FRAGMENT OF GCN4 ZIPPER CLONED N-TERMINAL TO A FRAGMENT OF GP41 Source: (gene. exp.) ![]() ![]() ![]() ![]() Genus: Lentivirus / Plasmid: PRSET / Gene: GCN4,AAS3,ARG9,YEL009C / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 4049.338 Da / Num. of mol.: 1 / Fragment: OUTER PEPTIDE OF GP41 OF HIV-1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide is naturally found in HIV-1. References: UniProt: P03377, UniProt: P04578*PLUS |
Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.29 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.9 Details: 25mM PIPES, 0.25M MgAc, 2.5% isopropanol, 10mM Hepes, 37.5mM NaCl, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: drop consists of equal amounts of protein and reservoir solutionspH: 8.3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 14, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 3→15 Å / Num. all: 2806 / Num. obs: 2574 / % possible obs: 88 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.072 |
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Processing
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Refinement | Resolution: 3→15 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber Details: Residue 125 chain C was modelled based on residues 124 and 126. The occupancy of this residue is 0.00.
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Refinement step | Cycle: LAST / Resolution: 3→15 Å
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