Journal: Biochemistry / Year: 2006 Title: Coiled Coils at the Edge of Configurational Heterogeneity. Structural Analyses of Parallel and Antiparallel Homotetrameric Coiled Coils Reveal Configurational Sensitivity to a Single Solvent- ...Title: Coiled Coils at the Edge of Configurational Heterogeneity. Structural Analyses of Parallel and Antiparallel Homotetrameric Coiled Coils Reveal Configurational Sensitivity to a Single Solvent-Exposed Amino Acid Substitution. Authors: Yadav, M.K. / Leman, L.J. / Price, D.J. / Brooks, C.L. / Stout, C.D. / Ghadiri, M.R.
Mass: 18.015 Da / Num. of mol.: 93 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED MUTATION IN CHAINS A, B, C, D: ARG 251 TO LYS ENGINEERED MUTATION IN CHAINS A, B, C, D: ...ENGINEERED MUTATION IN CHAINS A, B, C, D: ARG 251 TO LYS ENGINEERED MUTATION IN CHAINS A, B, C, D: ARG 256 TO LYS ENGINEERED MUTATION IN CHAINS A, B, C, D: CYS 268 TO GLU ENGINEERED MUTATION IN CHAINS A, B, C, D: ARG 275 TO LYS ENGINEERED MUTATION IN CHAINS A, B, C, D: ARG 276 TO LYS ENGINEERED MUTATION IN CHAINS A, B, C, D: TYR 265 TO HIS
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 3
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Sample preparation
Crystal
Density Matthews: 2 Å3/Da / Density % sol: 38.9 % Description: STRUCTURE WAS ORIGINALLY SOLVED BETWEEN SIRAS PHASING BETWEEN 2SEMET PEPTIDE AND IT'S DERIVATIVE SOAKED WITH 50MM KAUCN2
Crystal grow
Method: vapor diffusion, hanging drop / pH: 5 Details: HANGING DROP, 1UL OF 10MG/ML PEPTIDE IN WATER, 1UL 100MM CAPS, 30% PEG 400, PH 10.5, 1UL OF 50MM TCEP IN WATER