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Open data
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Basic information
Entry | Database: PDB / ID: 1gcl | ||||||
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Title | GCN4 LEUCINE ZIPPER CORE MUTANT P-LI | ||||||
![]() | GCN4 | ||||||
![]() | LEUCINE ZIPPER / HYDROPHOBIC CORE MUTANT | ||||||
Function / homology | ![]() protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding ...protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding / positive regulation of transcription initiation by RNA polymerase II / positive regulation of RNA polymerase II transcription preinitiation complex assembly / amino acid biosynthetic process / cellular response to amino acid starvation / RNA polymerase II transcription regulator complex / : / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Harbury, P.B. / Zhang, T. / Kim, P.S. / Alber, T. | ||||||
![]() | ![]() Title: A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants. Authors: Harbury, P.B. / Zhang, T. / Kim, P.S. / Alber, T. #1: ![]() Title: X-Ray Structure of the GCN4 Leucine Zipper, a Two-Stranded, Parallel Coiled Coil Authors: Shea, E.K. / Klemm, J.D. / Kim, P.S. / Alber, T. #2: ![]() Title: Evidence that the Leucine Zipper is a Coiled Coil Authors: Shea, E.K. / Rutkowski, R. / Kim, P.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.5 KB | Display | ![]() |
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PDB format | ![]() | 27.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 388.1 KB | Display | ![]() |
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Full document | ![]() | 399.5 KB | Display | |
Data in XML | ![]() | 5.7 KB | Display | |
Data in CIF | ![]() | 8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 4072.837 Da / Num. of mol.: 4 / Mutation: L5I,V9L,L12I,N16L,L19I,V23L,L26I,V30L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P03069 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.83 Å3/Da / Density % sol: 32.89 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 3 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.54 |
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Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Num. obs: 5643 / % possible obs: 73 % / Observed criterion σ(I): 2 |
Reflection | *PLUS Highest resolution: 2.1 Å / Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Highest resolution: 2.1 Å / σ(F): 5 /
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Displacement parameters | Biso mean: 40 Å2 | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.1 Å
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Refine LS restraints |
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