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- PDB-1uo2: Structure Based Engineering of Internal Molecular Surfaces Of Fou... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1uo2 | ||||||
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Title | Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles | ||||||
![]() | GENERAL CONTROL PROTEIN GCN4 | ||||||
![]() | FOUR HELIX BUNDLE / CAVITY | ||||||
Function / homology | ![]() protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding ...protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / nitrogen catabolite activation of transcription from RNA polymerase II promoter / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding / positive regulation of transcription initiation by RNA polymerase II / positive regulation of RNA polymerase II transcription preinitiation complex assembly / amino acid biosynthetic process / cellular response to amino acid starvation / RNA polymerase II transcription regulator complex / : / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Yadav, M.K. / Redman, J.E. / Alvarez-Gutierrez, J.M. / Zhang, Y. / Stout, C.D. / Ghadiri, M.R. | ||||||
![]() | ![]() Title: Structure-Based Engineering of Internal Cavities in Coiled-Coil Peptides Authors: Yadav, M.K. / Redman, J.E. / Leman, L.J. / Alvarez-Gutierrez, J.M. / Zhang, Y. / Stout, C.D. / Ghadiri, M.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 25.3 KB | Display | ![]() |
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PDB format | ![]() | 18.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 437.9 KB | Display | ![]() |
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Full document | ![]() | 437.8 KB | Display | |
Data in XML | ![]() | 5.2 KB | Display | |
Data in CIF | ![]() | 6.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1untC ![]() 1unuC ![]() 1unvC ![]() 1unwC ![]() 1unxC ![]() 1unyC ![]() 1unzC ![]() 1uo0C ![]() 1uo1C ![]() 1uo3C ![]() 1uo4C ![]() 1uo5C ![]() 1w5gC ![]() 1w5iC ![]() 2bniC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein/peptide | Mass: 4072.837 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: BASED ON SEQUENCE FROM SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) Source: (synth.) ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50 % |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 Details: HANGING DROP, RESERVOIR: 2.5 M NACL, 100 MM NAAC, 200 MM LI2SO4, PH 4.5 |
-Data collection
Diffraction | Mean temperature: 114 K |
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Diffraction source | Source: ![]() |
Detector | Date: Oct 15, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→35.58 Å / Num. obs: 6330 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 5.96 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 1.99→2.06 Å / Redundancy: 4.68 % / Rmerge(I) obs: 0.607 / Mean I/σ(I) obs: 1 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 46.016 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.99→55.9 Å
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