THE ASYMMETRIC UNIT CONTAINS ONE HALF OF PROTEIN/DNA COMPLEX PER ASYMMETRIC UNIT. MOLECULAR DYAD AXIS OF PROTEIN DIMER AND PALINDROMIC HALF SITES OF THE DNA COINCIDES WITH CRYSTALLOGRAPHIC TWO-FOLD AXIS. THE FULL PROTEIN/DNA COMPLEX CAN BE OBTAINED BY APPLYING THE FOLLOWING TRANSFORMATION MATRIX AND TRANSLATION VECTOR TO THE COORDINATES X Y Z: 0 -1 0 X 117.32 X SYMM -1 0 0 Y + 117.32 = Y SYMM 0 0 -1 Z 43.33 Z SYMM
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Components
#1: DNA chain
DNA (5'-D(*TP*GP*GP*AP*GP*AP*TP*GP*AP*CP*GP*TP*CP*AP*TP*CP*TP*CP*C)-3')
Mass: 5820.771 Da / Num. of mol.: 1 / Source method: obtained synthetically
#2: Protein
PROTEIN (GCN4)
Mass: 7244.369 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P03069
Sequence details
AMINO ACIDS NUMBERING (RESIDUE NUMBER) CORRESPONDS TO THE 281 AMINO ACIDS OF INTACT GCN4.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.8 Å3/Da / Density meas: 1.28 Mg/m3 / Density % sol: 56.14 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.25 Details: pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K
Components of the solutions
ID
Name
Crystal-ID
Sol-ID
1
WATER
1
1
2
PEG6000
1
1
3
NACITRATE
1
1
4
CACL2
1
1
5
WATER
1
2
6
PEG6000
1
2
7
NACITRATE
1
2
8
CACL2
1
2
Crystal grow
*PLUS
Temperature: 4 ℃ / Method: vapor diffusion, hanging drop
Components of the solutions
*PLUS
ID
Conc.
Common name
Crystal-ID
Sol-ID
Chemical formula
1
0.2-0.4 mM
protein
1
drop
2
oligonucleotide
1
drop
3
15mM
sodiumcitrate
1
drop
4
15mM
1
drop
CaCl2
5
9 %(w/v)
PEG6000
1
drop
6
30mM
sodiumcitrate
1
reservoir
7
30mM
1
reservoir
CaCl2
8
18 %
PEG6000
1
reservoir
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Data collection
Reflection
Resolution: 3→20 Å
Reflection
*PLUS
Highest resolution: 3 Å / Lowest resolution: 20 Å / % possible obs: 99 % / Rmerge(I) obs: 0.09
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