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- PDB-2wq1: GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating... -

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Basic information

Entry
Database: PDB / ID: 2wq1
TitleGCN4 leucine zipper mutant with three IxxNTxx motifs coordinating bromide
ComponentsGENERAL CONTROL PROTEIN GCN4
KeywordsTRANSCRIPTION / TAA / NUCLEUS / COILED COIL / DNA-BINDING / PROTEIN EXPORT / ION COORDINATION / POLAR CORE RESIDUES / TRIMERIC AUTOTRANSPORTER ADHESIN
Function / homology
Function and homology information


nitrogen catabolite activation of transcription from RNA polymerase II promoter / FCERI mediated MAPK activation / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / mediator complex binding / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding ...nitrogen catabolite activation of transcription from RNA polymerase II promoter / FCERI mediated MAPK activation / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / mediator complex binding / Oxidative Stress Induced Senescence / TFIID-class transcription factor complex binding / amino acid biosynthetic process / positive regulation of RNA polymerase II transcription preinitiation complex assembly / positive regulation of transcription initiation by RNA polymerase II / cellular response to nutrient levels / cellular response to amino acid starvation / RNA polymerase II transcription regulator complex / DNA-binding transcription activator activity, RNA polymerase II-specific / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription regulator complex / DNA-binding transcription factor activity, RNA polymerase II-specific / intracellular signal transduction / DNA-binding transcription factor activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus
Similarity search - Function
: / Basic region leucine zipper / Basic-leucine zipper (bZIP) domain signature. / Basic-leucine zipper (bZIP) domain profile. / basic region leucin zipper / Basic-leucine zipper domain / Basic-leucine zipper domain superfamily
Similarity search - Domain/homology
BROMIDE ION / General control transcription factor GCN4
Similarity search - Component
Biological speciesSACCHAROMYCES CEREVISIAE (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.08 Å
AuthorsHartmann, M.D. / Hernandez Alvarez, B. / Lupas, A.N.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2009
Title: A Coiled-Coil Motif that Sequesters Ions to the Hydrophobic Core.
Authors: Hartmann, M.D. / Ridderbusch, O. / Zeth, K. / Albrecht, R. / Testa, O. / Woolfson, D.N. / Sauer, G. / Dunin-Horkawicz, S. / Lupas, A.N. / Alvarez, B.H.
History
DepositionAug 12, 2009Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 3, 2009Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3May 8, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: GENERAL CONTROL PROTEIN GCN4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)4,2074
Polymers3,9671
Non-polymers2403
Water1,26170
1
A: GENERAL CONTROL PROTEIN GCN4
hetero molecules

A: GENERAL CONTROL PROTEIN GCN4
hetero molecules

A: GENERAL CONTROL PROTEIN GCN4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,62212
Polymers11,9023
Non-polymers7199
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation9_555y,z,x1
crystal symmetry operation5_555z,x,y1
Buried area3480 Å2
ΔGint-31.49 kcal/mol
Surface area6650 Å2
MethodPISA
Unit cell
Length a, b, c (Å)56.580, 56.580, 56.580
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number199
Space group name H-MI213
Components on special symmetry positions
IDModelComponents
11A-1033-

BR

21A-1034-

BR

31A-1035-

BR

41A-2010-

HOH

51A-2031-

HOH

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Components

#1: Protein/peptide GENERAL CONTROL PROTEIN GCN4 / AMINO ACID BIOSYNTHESIS REGULATORY PROTEIN / GCN4 LEUCINE ZIPPER MUTANT


Mass: 3967.468 Da / Num. of mol.: 1 / Fragment: COILED-COIL DOMAIN, RESIDUES 249-281 / Mutation: YES / Source method: obtained synthetically / Source: (synth.) SACCHAROMYCES CEREVISIAE (brewer's yeast) / References: UniProt: P03069
#2: Chemical ChemComp-BR / BROMIDE ION


Mass: 79.904 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Br
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 70 / Source method: isolated from a natural source / Formula: H2O
Compound detailsENGINEERED RESIDUE IN CHAIN A, VAL 257 TO ILE ENGINEERED RESIDUE IN CHAIN A, LEU 260 TO ASN ...ENGINEERED RESIDUE IN CHAIN A, VAL 257 TO ILE ENGINEERED RESIDUE IN CHAIN A, LEU 260 TO ASN ENGINEERED RESIDUE IN CHAIN A, LEU 261 TO THR ENGINEERED RESIDUE IN CHAIN A, ASN 264 TO ILE ENGINEERED RESIDUE IN CHAIN A, LEU 267 TO ASN ENGINEERED RESIDUE IN CHAIN A, GLU 268 TO THR ENGINEERED RESIDUE IN CHAIN A, VAL 271 TO ILE ENGINEERED RESIDUE IN CHAIN A, LEU 274 TO ASN ENGINEERED RESIDUE IN CHAIN A, LYS 275 TO THR

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2 Å3/Da / Density % sol: 39 % / Description: NONE
Crystal growDetails: 20% (W/V) PEG 3350, 200 MM NABR, 100 MM BIS-TRIS PROPANE PH 8.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.9184
DetectorType: MARRESEARCH / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9184 Å / Relative weight: 1
ReflectionResolution: 1.08→18 Å / Num. obs: 13026 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 4.11 % / Biso Wilson estimate: 12 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 10
Reflection shellResolution: 1.08→1.15 Å / Redundancy: 3.97 % / Rmerge(I) obs: 0.66 / Mean I/σ(I) obs: 2.01 / % possible all: 98.8

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Processing

Software
NameVersionClassification
REFMAC5.2.0019refinement
XDSdata reduction
XSCALEdata scaling
SHELXphasing
RefinementMethod to determine structure: SAD
Starting model: NONE

Resolution: 1.08→16.33 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.959 / SU B: 0.913 / SU ML: 0.021 / Cross valid method: THROUGHOUT / ESU R: 0.032 / ESU R Free: 0.035 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
RfactorNum. reflection% reflectionSelection details
Rfree0.16748 657 5.1 %RANDOM
Rwork0.12741 ---
obs0.12938 12350 99.85 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parametersBiso mean: 11.58 Å2
Refinement stepCycle: LAST / Resolution: 1.08→16.33 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms256 0 3 70 329
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0190.021366
X-RAY DIFFRACTIONr_bond_other_d0.0030.02247
X-RAY DIFFRACTIONr_angle_refined_deg1.8341.968495
X-RAY DIFFRACTIONr_angle_other_deg1.2593620
X-RAY DIFFRACTIONr_dihedral_angle_1_deg4.897545
X-RAY DIFFRACTIONr_dihedral_angle_2_deg36.99528.09521
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.3851584
X-RAY DIFFRACTIONr_dihedral_angle_4_deg11.299151
X-RAY DIFFRACTIONr_chiral_restr0.1370.258
X-RAY DIFFRACTIONr_gen_planes_refined0.0080.02402
X-RAY DIFFRACTIONr_gen_planes_other0.0030.0255
X-RAY DIFFRACTIONr_nbd_refined0.3850.283
X-RAY DIFFRACTIONr_nbd_other0.2110.2220
X-RAY DIFFRACTIONr_nbtor_refined0.1840.2126
X-RAY DIFFRACTIONr_nbtor_other0.0920.2174
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.170.240
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.0910.216
X-RAY DIFFRACTIONr_symmetry_vdw_other0.2810.230
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1910.215
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it5.23724254
X-RAY DIFFRACTIONr_mcbond_other3.9752491
X-RAY DIFFRACTIONr_mcangle_it5.73232369
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it6.88948145
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it8.8272125
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr2.9243658
X-RAY DIFFRACTIONr_sphericity_free8.898373
X-RAY DIFFRACTIONr_sphericity_bonded6.0263608
LS refinement shellResolution: 1.082→1.11 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.258 48 -
Rwork0.203 921 -
obs--100 %

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