2C8Q

insuline(1sec) and UV laser excited fluorescence

2C8Q の概要

• エントリーDOI: 10.2210/pdb2c8q/pdb
• 関連するPDBエントリー: 1A7F 1AI0 1AIY 1B9E 1BEN 1EFE 1EV3 1EV6 1EVR 1FU2 1FUB 1G7A 1G7B 1GUJ 1HIQ 1HIS 1HIT 1HLS 1HTV 1HUI 1IOG 1IOH 1J73 1JCA 1JCO 1K3M 1KMF 1LKQ 1LNP 1LPH 1MHI 1MHJ 1MSO 1OS3 1OS4 1Q4V 1QIY 1QIZ 1QJ0 1RWE 1SF1 1SJT 1SJU 1T0C 1T1K 1T1P 1T1Q 1TRZ 1TYL 1TYM 1UZ9 1VKT 1W8P 1XDA 1XGL 1XW7 1ZEG 1ZEH 1ZNJ 2AIY 2C8R 2HIU 3AIY 4AIY 5AIY
• 分子名称: INSULIN A CHAIN, INSULIN B CHAIN (3 entities in total)
• 機能のキーワード: laser, uv, carbohydrate metabolism, hormone, diabetes mellitus, glucose metabolism
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Secreted: P01308 P01308
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5716.55

構造登録者

Vernede, X.,Lavault, B.,Ohana, J.,Nurizzo, D.,Joly, J.,Jacquamet, L.,Felisaz, F.,Cipriani, F.,Bourgeois, D. (登録日: 2005-12-06, 公開日: 2006-03-08, 最終更新日: 2023-12-13)

主引用文献

Vernede, X.,Lavault, B.,Ohana, J.,Nurizzo, D.,Joly, J.,Jacquamet, L.,Felisaz, F.,Cipriani, F.,Bourgeois, D.
Uv Laser-Excited Fluorescence as a Tool for the Visualization of Protein Crystals Mounted in Loops.
Acta Crystallogr.,Sect.D, 62:253-, 2006

PubMed Abstract: Structural proteomics has promoted the rapid development of automated protein structure determination using X-ray crystallography. Robotics are now routinely used along the pipeline from genes to protein structures. However, a bottleneck still remains. At synchrotron beamlines, the success rate of automated sample alignment along the X-ray beam is limited by difficulties in visualization of protein crystals, especially when they are small and embedded in mother liquor. Despite considerable improvement in optical microscopes, the use of visible light transmitted or reflected by the sample may result in poor or misleading contrast. Here, the endogenous fluorescence from aromatic amino acids has been used to identify even tiny or weakly fluorescent crystals with a high success rate. The use of a compact laser at 266 nm in combination with non-fluorescent sample holders provides an efficient solution to collect high-contrast fluorescence images in a few milliseconds and using standard camera optics. The best image quality was obtained with direct illumination through a viewing system coaxial with the UV beam. Crystallographic data suggest that the employed UV exposures do not generate detectable structural damage.

PubMed: 16510972
DOI: 10.1107/S0907444905041429

実験手法

X-RAY DIFFRACTION (1.95 Å)