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- PDB-2ye0: X-ray structure of the cyan fluorescent protein mTurquoise (K206A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ye0 | ||||||
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Title | X-ray structure of the cyan fluorescent protein mTurquoise (K206A mutant) | ||||||
![]() | GREEN FLUORESCENT PROTEIN | ||||||
![]() | FLUORESCENT PROTEIN / FRET DONOR | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | von Stetten, D. / Goedhart, J. / Noirclerc-Savoye, M. / Lelimousin, M. / Joosen, L. / Hink, M.A. / van Weeren, L. / Gadella, T.W.J. / Royant, A. | ||||||
![]() | ![]() Title: Structure-Guided Evolution of Cyan Fluorescent Proteins Towards a Quantum Yield of 93% Authors: Goedhart, J. / von Stetten, D. / Noirclerc-Savoye, M. / Lelimousin, M. / Joosen, L. / Hink, M.A. / van Weeren, L. / Gadella, T.W.J. / Royant, A. #1: Journal: Nat.Methods / Year: 2010 Title: Bright Cyan Fluorescent Protein Variants Identified by Fluorescence Lifetime Screening. Authors: Goedhart, J. / van Weeren, L. / Hink, M.A. / Vischer, N.O.E. / Jalink, K. / Gadella, T.W.J.J. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.2 KB | Display | ![]() |
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PDB format | ![]() | 87.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.8 KB | Display | ![]() |
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Full document | ![]() | 424.3 KB | Display | |
Data in XML | ![]() | 12.2 KB | Display | |
Data in CIF | ![]() | 17.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ydzC ![]() 3ztfC ![]() 2wsoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27666.162 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
Compound details | ENGINEERED RESIDUE IN CHAIN A, PHE 64 TO LEU ENGINEERED RESIDUE IN CHAIN A, TYR 66 TO TRP ...ENGINEERED |
Sequence details | CHROMOPHOR |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 39 % Description: DATA WERE RECORDED USING THE ESRF HELICAL DATA COLLECTION PROCEDURE. |
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Crystal grow | pH: 6.75 Details: 16 MG/ML PROTEIN, 14% PEG8000, 100 MM MGCL2, 100 MM HEPES PH 6.75 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 30, 2010 / Details: TOROIDAL MIRROR |
Radiation | Monochromator: SI111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 1.47→46.7 Å / Num. obs: 38321 / % possible obs: 98.8 % / Observed criterion σ(I): 2 / Redundancy: 4.1 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 15.9 |
Reflection shell | Resolution: 1.47→1.55 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.75 / Mean I/σ(I) obs: 2 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2WSO Resolution: 1.47→41.29 Å / Cor.coef. Fo:Fc: 0.975 / Cor.coef. Fo:Fc free: 0.968 / SU B: 2.509 / SU ML: 0.042 / Cross valid method: THROUGHOUT / ESU R: 0.07 / ESU R Free: 0.065 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.835 Å2
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Refinement step | Cycle: LAST / Resolution: 1.47→41.29 Å
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Refine LS restraints |
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