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Open data
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Basic information
Entry | Database: PDB / ID: 1bfp | ||||||
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Title | BLUE VARIANT OF GREEN FLUORESCENT PROTEIN | ||||||
![]() | BLUE FLUORESCENT PROTEIN | ||||||
![]() | LUMINESCENCE / FLUORESCENT PROTEIN / BLUE EMISSION / MUTANT / FLUOROPHORE / BIOLUMINESCENSE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wachter, R.M. / Remington, S.J. | ||||||
![]() | ![]() Title: Crystal structure and photodynamic behavior of the blue emission variant Y66H/Y145F of green fluorescent protein. Authors: Wachter, R.M. / King, B.A. / Heim, R. / Kallio, K. / Tsien, R.Y. / Boxer, S.G. / Remington, S.J. #1: ![]() Title: Engineering Green Fluorescent Protein for Improved Brightness, Longer Wavelengths and Fluorescence Resonance Energy Transfer Authors: Heim, R. / Tsien, R.Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.2 KB | Display | ![]() |
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PDB format | ![]() | 42.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 371.5 KB | Display | ![]() |
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Full document | ![]() | 382.6 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 11.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1emaS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26891.338 Da / Num. of mol.: 1 / Mutation: S65, H66, AND G67 ARE REPLACED WITH IIC 66, Y145F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.7 % / Description: ISOMORPHOUS REPLACEMENT | |||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / pH: 4.5 Details: PROTEIN WAS CRYSTALLIZED AT 4 DEGC FROM 100MM SODIUM ACETATE PH 4.5 AND 10-12% PEG3400., temperature 277K | |||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Sep 19, 1996 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 12044 / % possible obs: 87 % / Observed criterion σ(I): 0 / Redundancy: 2.64 % / Biso Wilson estimate: 19.7 Å2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 2.1→2.26 Å / Redundancy: 1.3 % / Rmerge(I) obs: 0.14 / Mean I/σ(I) obs: 2.24 / % possible all: 64 |
Reflection | *PLUS Num. measured all: 31786 |
Reflection shell | *PLUS % possible obs: 65 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EMA Resolution: 2.1→20 Å / Isotropic thermal model: TNT / σ(F): 0 / Stereochemistry target values: TNT
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Solvent computation | Bsol: 300 Å2 / ksol: 0.8 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5F / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.181 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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