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- PDB-2hqz: Crystal structure of L42H design intermediate for GFP metal ion r... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2hqz | ||||||
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Title | Crystal structure of L42H design intermediate for GFP metal ion reporter | ||||||
![]() | Green fluorescent protein | ||||||
![]() | LUMINESCENT PROTEIN / metal site design / intermediate / GFP / fluorophore / reporter | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Barondeau, D.P. / Tubbs, J.L. / Tainer, J.A. / Getzoff, E.D. | ||||||
![]() | ![]() Title: Iterative Structure-Based Design of a Green Fluorescent Protein Metal Ion Reporter Authors: Barondeau, D.P. / Tubbs, J.L. / Tainer, J.A. / Getzoff, E.D. | ||||||
History |
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Remark 999 | SEQUENCE Residue Ser 65 has been mutated to Thr. Residues Ser 65, Tyr 66 and Gly 67 constitute the ...SEQUENCE Residue Ser 65 has been mutated to Thr. Residues Ser 65, Tyr 66 and Gly 67 constitute the chromophore CRO |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.4 KB | Display | ![]() |
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PDB format | ![]() | 49.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.8 KB | Display | ![]() |
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Full document | ![]() | 448.3 KB | Display | |
Data in XML | ![]() | 15.7 KB | Display | |
Data in CIF | ![]() | 23.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2hjoC ![]() 2hrsC ![]() 1emaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26907.291 Da / Num. of mol.: 1 / Mutation: F64L, S65T, L42H Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.9 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 19% PEG 4K, 50 mM MgCl2, 50 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Dec 20, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→30 Å / Num. obs: 71363 / % possible obs: 98.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Biso Wilson estimate: 11.4 Å2 / Rsym value: 0.055 / Net I/σ(I): 23.7 |
Reflection shell | Resolution: 1.2→1.24 Å / Mean I/σ(I) obs: 3.6 / Num. unique all: 6908 / Rsym value: 0.351 / % possible all: 96.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EMA Resolution: 1.2→30 Å / Cross valid method: thoughout / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.2→30 Å
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Refine LS restraints |
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