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- PDB-1jbz: CRYSTAL STRUCTURE ANALYSIS OF A DUAL-WAVELENGTH EMISSION GREEN FL... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jbz | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF A DUAL-WAVELENGTH EMISSION GREEN FLUORESCENT PROTEIN VARIANT AT HIGH PH | ||||||
![]() | GREEN FLUORESCENT PROTEIN | ||||||
![]() | LUMINESCENT PROTEIN / Beta Barrel / Chromophore | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hanson, G.T. / McAnaney, T.B. / Park, E.S. / Rendell, M.E.P. / Yarbrough, D.K. / Chu, S. / Xi, L. / Boxer, S.G. / Montrose, M.H. / Remington, S.J. | ||||||
![]() | ![]() Title: Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 1. Structural Characterization and Preliminary Application. Authors: Hanson, G.T. / McAnaney, T.B. / Park, E.S. / Rendell, M.E.P. / Yarbrough, D.K. / Chu, S. / Xi, L. / Boxer, S.G. / Montrose, M.H. / Remington, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.3 KB | Display | ![]() |
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PDB format | ![]() | 45.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1jbyC ![]() 1emaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 26866.326 Da / Num. of mol.: 1 / Mutation: S65T,Q80R,H148G,T203C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | RESIDUES 65, 66, AND 67 ARE NOT PRESENT IN THE ENTRY AND ARE INSTEAD REPLACED WITH CRO 66 | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 9 Details: PEG 4000, Tris, Magnesium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.9 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 27, 2000 Details: 58 cm long, Pt-coated fused silica, vertical focus mirror |
Radiation | Monochromator: Cylindrically bent triangular Si(111) asymmetric cut horizontal focus Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→19.9 Å / Num. all: 120478 / Num. obs: 34536 / % possible obs: 96.2 % / Redundancy: 3.5 % / Biso Wilson estimate: 16.6 Å2 / Rmerge(I) obs: 0.045 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 1.5→1.57 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.319 / Mean I/σ(I) obs: 2.3 / Num. unique all: 4801 / % possible all: 93.6 |
Reflection | *PLUS Num. measured all: 120478 |
Reflection shell | *PLUS Lowest resolution: 1.58 Å / % possible obs: 93.6 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EMA Resolution: 1.5→19.9 Å / Stereochemistry target values: ENGH & HUBER /
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Refinement step | Cycle: LAST / Resolution: 1.5→19.9 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.57 Å /
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Refinement | *PLUS Rfactor all: 0.177 | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
Refine LS restraints | *PLUS
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