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- PDB-1jc0: CRYSTAL STRUCTURE ANALYSIS OF A REDOX-SENSITIVE GREEN FLUORESCENT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jc0 | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF A REDOX-SENSITIVE GREEN FLUORESCENT PROTEIN VARIANT IN A REDUCED FORM | ||||||
![]() | GREEN FLUORESCENT PROTEIN | ||||||
![]() | LUMINESCENT PROTEIN / Beta Barrel / Chromophore | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hanson, G.T. / Aggeler, R. / Oglesbee, D. / Cannon, M. / Capaldi, R.A. / Tsien, R.Y. / Remington, S.J. | ||||||
![]() | ![]() Title: Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. Authors: Hanson, G.T. / Aggeler, R. / Oglesbee, D. / Cannon, M. / Capaldi, R.A. / Tsien, R.Y. / Remington, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 147.1 KB | Display | ![]() |
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PDB format | ![]() | 114.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.9 KB | Display | ![]() |
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Full document | ![]() | 540.5 KB | Display | |
Data in XML | ![]() | 38.7 KB | Display | |
Data in CIF | ![]() | 51.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1jc1C ![]() 1emaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26886.379 Da / Num. of mol.: 3 / Mutation: C48S,F64L,S65T,Q80R,S147C,Q204C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | RESIDUES 65, 66, AND 67 ARE NOT PRESENT IN THE ENTRY AND ARE INSTEAD REPLACED WITH CRO 66. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.13 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, Lithium Sulfate, Tris, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.9 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 26, 2000 Details: 58 cm long, Pt-coated fused silica, vertical focus mirror |
Radiation | Monochromator: Cylindrically bent triangular Si(111) asymmetric cut horizontal focus Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2→42.3 Å / Num. all: 194884 / Num. obs: 38346 / % possible obs: 78.2 % / Redundancy: 5.1 % / Biso Wilson estimate: 29.4 Å2 / Rmerge(I) obs: 0.053 / Net I/σ(I): 8.7 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.335 / Mean I/σ(I) obs: 2.2 / Num. unique all: 5207 / % possible all: 74.3 |
Reflection | *PLUS Lowest resolution: 28.7 Å / Num. measured all: 194884 |
Reflection shell | *PLUS % possible obs: 74.3 % / Rmerge(I) obs: 0.304 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EMA Resolution: 2→30 Å / Stereochemistry target values: ENGH & HUBER /
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Refinement step | Cycle: LAST / Resolution: 2→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.1 Å
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Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. reflection obs: 36454 / Num. reflection Rfree: 3593 / Rfactor Rfree: 0.224 / Rfactor Rwork: 0.329 | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
Refine LS restraints | *PLUS
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