+
Open data
-
Basic information
Entry | Database: PDB / ID: 1q4c | ||||||
---|---|---|---|---|---|---|---|
Title | S65T Q80R T203C Green Fluorescent Protein (GFP) pH 8.5 | ||||||
![]() | Green Fluorescent Protein | ||||||
![]() | LUMINESCENT PROTEIN / green fluorescent protein / GFP / fluorophore / chromophore | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jain, R.K. / Ranganathan, R. | ||||||
![]() | ![]() Title: Local complexity of amino acid interactions in a protein core. Authors: Jain, R.K. / Ranganathan, R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 63.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 45.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1q4aC ![]() 1q4bC ![]() 1q4dC ![]() 1q4eC ![]() 1q73C ![]() 1emaS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 26947.422 Da / Num. of mol.: 1 / Mutation: Q80R, S65T, T203C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
Sequence details | RESIDUE SER A 65 IS MUTATED TO THR A 65. THR A 65, TYR A 66 AND GLY A 67 ARE MODIFIED TO MAKE ...RESIDUE SER A 65 IS MUTATED TO THR A 65. THR A 65, TYR A 66 AND GLY A 67 ARE MODIFIED TO MAKE CHROMOPHOR |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 34.95 % | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, MgCl2, BME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 8.5 / PH range high: 8.1 | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
---|---|
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.55→25 Å / Num. all: 31021 / Num. obs: 31021 / % possible obs: 95.3 % |
Reflection | *PLUS Lowest resolution: 24 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.057 |
Reflection shell | *PLUS % possible obs: 92.4 % / Rmerge(I) obs: 0.423 / Mean I/σ(I) obs: 2.5 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EMA Resolution: 1.55→25 Å / σ(F): 0
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.55→25 Å
| |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|