Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta Similarity search - Domain/homology
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA", "BA", "CA" IN CHAIN A, B, C ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA", "BA", "CA" IN CHAIN A, B, C RESPECTIVELY ON SHEET RECORDS BELOW ARE ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 840 / Source method: isolated from a natural source / Formula: H2O
Compound details
CHAIN A, B, C ENGINEERED MUTATION SER65ALA,CYS48VAL,VAL68LEU, ...CHAIN A, B, C ENGINEERED MUTATION SER65ALA,CYS48VAL,VAL68LEU, SER72ALA,GLN80ARG,ASN149CYS,MET153VAL,SER202CYS,THR203TYR, ASP234HIS
Sequence details
MODRES: 1H6R CRO() 66() THE FLUOROPHORE IS GENERATED BY AN AUTOCATALYTIC CYCLIZATION OF THE ...MODRES: 1H6R CRO() 66() THE FLUOROPHORE IS GENERATED BY AN AUTOCATALYTIC CYCLIZATION OF THE POLYPEPTIDE BACKBONE OF ALA 65, TYR 66, GLY 67. RESIDUES 65, 66, 67 ARE NOT PRESENT IN THE ENTRY, ARE INSTEAD REPLACED WITH PIA 66.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.5 Å3/Da / Density % sol: 49 %
Crystal grow
pH: 8 Details: 100 MM HEPES, PH 8.0, 100 MM MGCL2, AND 14% PEG4000
Crystal grow
*PLUS
Temperature: 22 ℃ / Method: vapor diffusion, hanging drop
Components of the solutions
*PLUS
ID
Conc.
Common name
Crystal-ID
Sol-ID
Details
Chemical formula
1
24mg/ml
protein
1
drop
2
10mM
HEPES
1
drop
pH8.0
3
100mM
HEPES
1
reservoir
pH8.0
4
100mM
1
reservoir
MgCl2
5
14 %(w/v)
PEG4000
1
reservoir
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: MAX II / Beamline: I711 / Wavelength: 1.0362
Resolution: 1.5→78.09 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 689676.36 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MLF Details: A SIGNIFICANT 2FO-FC AND FO-FC DENSITY WAS OBSERVED WITH A CENTER 1.5 ANGSTROM FROM CYS 70 SG AND IT IS POSSIBLE THAT CYS 70 IS IN AN OXIDIZED STATE.
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