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- PDB-5oxa: Structure of the S205A mutant of the Cyan Fluorescent Protein Cer... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5oxa | ||||||
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Title | Structure of the S205A mutant of the Cyan Fluorescent Protein Cerulean at pH 7.0 | ||||||
![]() | Green fluorescent protein | ||||||
![]() | FLUORESCENT PROTEIN / SCFP3A / chromophore / fluorescence | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gotthard, G. / von Stetten, D. / Clavel, D. / Noirclerc-Savoye, M. / Royant, A. | ||||||
![]() | ![]() Title: Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins. Authors: Gotthard, G. / von Stetten, D. / Clavel, D. / Noirclerc-Savoye, M. / Royant, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.3 KB | Display | ![]() |
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PDB format | ![]() | 105.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.7 KB | Display | ![]() |
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Full document | ![]() | 433.5 KB | Display | |
Data in XML | ![]() | 16.2 KB | Display | |
Data in CIF | ![]() | 25.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5ox8C ![]() 5ox9C ![]() 5oxbC ![]() 5oxcC ![]() 2wsoS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26775.252 Da / Num. of mol.: 1 / Mutation: F64L, S65T, Y66W, N146I, H148D, M153T, S205A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.14 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 100mM HEPES buffer pH 7.0, 12% PEG8000, 100mM MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 6, 2014 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.977178 Å / Relative weight: 1 |
Reflection | Resolution: 1.16→46.76 Å / Num. obs: 78153 / % possible obs: 99.9 % / Redundancy: 7.06 % / Rrim(I) all: 0.036 / Net I/σ(I): 24.64 |
Reflection shell | Resolution: 1.16→1.19 Å / Redundancy: 6.35 % / Mean I/σ(I) obs: 2.12 / Num. unique obs: 5697 / Rrim(I) all: 0.885 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2WSO Resolution: 1.16→46.75 Å / Cor.coef. Fo:Fc: 0.985 / Cor.coef. Fo:Fc free: 0.983 / SU B: 1.248 / SU ML: 0.024 / Cross valid method: THROUGHOUT / ESU R: 0.03 / ESU R Free: 0.03 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.356 Å2
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Refinement step | Cycle: 1 / Resolution: 1.16→46.75 Å
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Refine LS restraints |
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