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Structure paper

TitleChromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins.
Journal, issue, pagesBiochemistry, Vol. 56, Page 6418-6422, Year 2017
Publish dateSep 6, 2017 (structure data deposition date)
AuthorsGotthard, G. / von Stetten, D. / Clavel, D. / Noirclerc-Savoye, M. / Royant, A.
External linksBiochemistry / PubMed:29148725
MethodsX-ray diffraction
Resolution1.02 - 1.56 Å
Structure data

PDB-5ox8:
Structure of Enhanced Cyan Fluorescent Protein at pH 5.0
Method: X-RAY DIFFRACTION / Resolution: 1.29 Å

PDB-5ox9:
Structure of the Cyan Fluorescent Protein SCFP3A at pH 4.5
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-5oxa:
Structure of the S205A mutant of the Cyan Fluorescent Protein Cerulean at pH 7.0
Method: X-RAY DIFFRACTION / Resolution: 1.16 Å

PDB-5oxb:
Structure of blue-light irradiated Cerulean
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

PDB-5oxc:
Structure of Cerulean Fluorescent Protein at 1.02 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.02 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / tryptophan-based chromophore / ECFP / SCFP3A / chromophore / fluorescence / Cerulean / photoisomerization / hydrogen atoms

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