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- PDB-1f09: CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f09 | ||||||
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Title | CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT YFP-H148Q WITH TWO BOUND IODIDES | ||||||
![]() | GREEN FLUORESCENT PROTEIN | ||||||
![]() | LUMINESCENT PROTEIN / beta barrel / LUMINESCENCE / BIOLUMINESCENCE / PHOTOACTIVE PROTEIN / GREEN FLUORESCENT PROTEIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Wachter, R.M. / Yarbrough, D. / Kallio, K. / Remington, S.J. | ||||||
![]() | ![]() Title: Crystallographic and energetic analysis of binding of selected anions to the yellow variants of green fluorescent protein. Authors: Wachter, R.M. / Yarbrough, D. / Kallio, K. / Remington, S.J. #1: ![]() Title: Sensitivity of the Yellow Variant of Green Fluorescent Protein to Halides and Nitrate Authors: Wachter, R.M. / Remington, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.2 KB | Display | ![]() |
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PDB format | ![]() | 44.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.6 KB | Display | ![]() |
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Full document | ![]() | 449.4 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 20.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26951.408 Da / Num. of mol.: 1 / Mutation: S65G, V68L, S72A, Q80R, T203Y, H148Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 39.87 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: PEG 1550, sodium acetate, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7.9 | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Aug 30, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→30.1 Å / Num. all: 35882 / Num. obs: 10849 / % possible obs: 85.6 % / Redundancy: 3.31 % / Biso Wilson estimate: 27.6 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.519 / Num. unique all: 990 / % possible all: 79.6 |
Reflection | *PLUS Num. measured all: 35882 |
Reflection shell | *PLUS % possible obs: 79.6 % |
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Processing
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Refinement | Resolution: 2.14→30.1 Å / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.14→30.1 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||
Refinement | *PLUS Rfactor all: 0.204 | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS Type: t_angle_deg / Dev ideal: 2.83 |