2WBY

Crystal structure of human insulin-degrading enzyme in complex with insulin

Summary for 2WBY

Related1HIT 2JBU 2HHO 2C8Q 1TYL 2C8R 1T1K 1AIY 1XDA 1HTV 1MSO 1UZ9 1FUB 1TYM 1HUI 1VKT 2VK0 1T1Q 1HLS 2CEU 1QJ0 1MHJ 1FU2 1SJT 1QIY 1IOG 2VJZ 1IOH 1TRZ 1EVR 1EV3 1RWE 1OS4 1GUJ 1AI0 1JCO 1SF1 1JCA 1ZEG 1OS3 1XGL 1QIZ 1T0C 1G7B 2AIY 1EV6 1Q4V 2HH4 2H67 4AIY 1J73 1K3M 1MHI 2WC0 2HIU 1KMF 1XW7 5AIY 1G7A 1ZNJ 1ZEH 1HIS 1B9E 1W8P 3AIY 1HIQ 1EFE 1LPH 1T1P 1A7F 1BEN 2JG4 1LKQ
DescriptorINSULIN-DEGRADING ENZYME, INSULIN A CHAIN, INSULIN B CHAIN, ... (5 entities in total)
Functional Keywordshydrolase/hormone, glucose metabolism, carbohydrate metabolism, disease mutation, diabetes mellitus, zinc, dioxane, insulin, hormone, secreted, protease, disulfide bond, pharmaceutical, metalloprotease, human insulin-degradng enzyme, hydrolase, cytoplasm, polymorphism, metal-binding, cleavage on pair of basic residues, hydrolase-hormone complex
Biological sourceHOMO SAPIENS (HUMAN)
Cellular locationCytoplasm P14735
Secreted P01308 P01308
Total number of polymer chains6
Total molecular weight238086.2
Authors
Manolopoulou, M.,Guo, Q.,Malito, E.,Schilling, A.B.,Tang, W.J. (deposition date: 2009-03-06, release date: 2009-03-24, Last modification date: 2011-07-13)
Primary citation
Manolopoulou, M.,Guo, Q.,Malito, E.,Schilling, A.B.,Tang, W.J.
Molecular Basis of Catalytic Chamber-Assisted Unfolding and Cleavage of Human Insulin by Human Insulin Degrading Enzyme.
J.Biol.Chem., 284:14177-, 2009
PubMed: 19321446 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M900068200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.6 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.220100.1%9.8%1.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report