2WBY

Crystal structure of human insulin-degrading enzyme in complex with insulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	287
Detector technology	CCD
Collection date	2007-06-11
Spacegroup name	P 65
Unit cell lengths	262.319, 262.319, 90.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.080 - 2.600
R-factor	0.167
Rwork	0.164
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g47
RMSD bond length	0.024
RMSD bond angle	2.101
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.520
Number of reflections	109017
<I/σ(I)>	21	4.8
Completeness [%]	99.9	100
Redundancy	7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE