1.88 Angstrom Resolution Crystal Structure Holliday Junction ATP-dependent DNA Helicase (RuvB) from Pseudomonas aeruginosa in Complex with ADP
Components
Holliday junction ATP-dependent DNA helicase RuvB
Keywords
HYDROLASE / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / Holliday Junction ATP-dependent DNA Helicase / ADP
Function / homology
Function and homology information
cellular response to chromate / cellular response to selenite ion / Holliday junction resolvase complex / four-way junction helicase activity / four-way junction DNA binding / DNA recombination / DNA helicase / DNA repair / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 191 / Source method: isolated from a natural source / Formula: H2O
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.04 Å3/Da / Density % sol: 39.7 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 8.6 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris, pH 8.3, screen: Classics II (D3), 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine ED-2001
Resolution: 1.88→28.64 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.948 / SU B: 6.796 / SU ML: 0.1 / Cross valid method: THROUGHOUT / ESU R: 0.147 / ESU R Free: 0.136 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.21307
1295
5 %
RANDOM
Rwork
0.17247
-
-
-
obs
0.17452
24830
99.94 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å