Author results

2PUX
  • Download 2pux
  • View 2pux
Molmil generated image of 2pux
CRYSTAL STRUCTURE OF MURINE THROMBIN IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF MURINE PAR3
Descriptor:Thrombin light chain, Thrombin heavy chain, Proteinase-activated receptor 3, ...
Authors:Bah, A., Chen, Z., Bush-Pelc, L.A., Mathews, F.S., Di Cera, E.
Deposit date:2007-05-09
Release date:2007-07-10
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Crystal structures of murine thrombin in complex with the extracellular fragments of murine protease-activated receptors PAR3 and PAR4.
Proc.Natl.Acad.Sci.Usa, 104, 2007
2PV9
  • Download 2pv9
  • View 2pv9
Molmil generated image of 2pv9
CRYSTAL STRUCTURE OF MURINE THROMBIN IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF MURINE PAR4
Descriptor:Thrombin light chain, Thrombin heavy chain, Proteinase-activated receptor 4, ...
Authors:Bah, A., Chen, Z., Bush-Pelc, L.A., Mathews, F.S., Di Cera, E.
Deposit date:2007-05-09
Release date:2007-07-10
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (3.5 Å)
Cite:Crystal structures of murine thrombin in complex with the extracellular fragments of murine protease-activated receptors PAR3 and PAR4.
Proc.Natl.Acad.Sci.Usa, 104, 2007
3BEF
  • Download 3bef
  • View 3bef
Molmil generated image of 3bef
CRYSTAL STRUCTURE OF THROMBIN BOUND TO THE EXTRACELLULAR FRAGMENT OF PAR1
Descriptor:Prothrombin, Proteinase-activated receptor 1, N-ACETYL-D-GLUCOSAMINE
Authors:Gandhi, P.S., Bah, A., Chen, Z., Mathews, F.S., Di Cera, E.
Deposit date:2007-11-17
Release date:2008-01-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Structural identification of the pathway of long-range communication in an allosteric enzyme.
Proc.Natl.Acad.Sci.Usa, 105, 2008
3JZ1
  • Download 3jz1
  • View 3jz1
Molmil generated image of 3jz1
CRYSTAL STRUCTURE OF HUMAN THROMBIN MUTANT N143P IN E:NA+ FORM
Descriptor:Thrombin light chain, Thrombin heavy chain, SODIUM ION, ...
Authors:Niu, W., Chen, Z., Bush-Pelc, L.A., Bah, A., Gandhi, P.S., Di Cera, E.
Deposit date:2009-09-22
Release date:2009-10-20
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Mutant N143P reveals how Na+ activates thrombin
J.Biol.Chem., 284, 2009
3JZ2
  • Download 3jz2
  • View 3jz2
Molmil generated image of 3jz2
CRYSTAL STRUCTURE OF HUMAN THROMBIN MUTANT N143P IN E* FORM
Descriptor:Thrombin light chain, Thrombin heavy chain, N-ACETYL-D-GLUCOSAMINE, ...
Authors:Niu, W., Chen, Z., Bush-Pelc, L.A., Bah, A., Gandhi, P.S., Di Cera, E.
Deposit date:2009-09-22
Release date:2009-10-20
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Mutant N143P reveals how Na+ activates thrombin
J.Biol.Chem., 284, 2009
4DT7
  • Download 4dt7
  • View 4dt7
Molmil generated image of 4dt7
CRYSTAL STRUCTURE OF THROMBIN BOUND TO THE ACTIVATION DOMAIN QEDQVDPRLIDGKMTRRGDS OF PROTEIN C
Descriptor:Thrombin light chain, Thrombin heavy chain, Vitamin K-dependent protein C, ...
Authors:Pozzi, N., Barranco-Medina, S., Chen, Z., Di Cera, E.
Deposit date:2012-02-20
Release date:2012-05-09
Last modified:2012-08-15
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Exposure of R169 controls protein C activation and autoactivation.
Blood, 120, 2012
3QDZ
  • Download 3qdz
  • View 3qdz
Molmil generated image of 3qdz
CRYSTAL STRUCTURE OF THE HUMAN THROMBIN MUTANT D102N IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF HUMAN PAR4.
Descriptor:Thrombin light chain, Thrombin heavy chain, Proteinase-activated receptor 4
Authors:Gandhi, P., Chen, Z., Appelbaum, E., Zapata, F., Di Cera, E.
Deposit date:2011-01-19
Release date:2011-06-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Structural basis of thrombin-protease-receptor interactions
IUBMB LIFE, 63, 2011
4NZQ
  • Download 4nzq
  • View 4nzq
Molmil generated image of 4nzq
CRYSTAL STRUCTURE OF CA2+-FREE PROTHROMBIN DELETION MUTANT RESIDUES 146-167
Descriptor:Prothrombin, N-ACETYL-D-GLUCOSAMINE
Authors:Pozzi, N., Chen, Z., Shropshire, D.B., Pelc, L.A., Di Cera, E.
Deposit date:2013-12-12
Release date:2014-05-21
Last modified:2017-08-23
Method:X-RAY DIFFRACTION (2.807 Å)
Cite:The linker connecting the two kringles plays a key role in prothrombin activation.
Proc.Natl.Acad.Sci.USA, 111, 2014
2GP9
  • Download 2gp9
  • View 2gp9
Molmil generated image of 2gp9
CRYSTAL STRUCTURE OF THE SLOW FORM OF THROMBIN IN A SELF-INHIBITED CONFORMATION
Descriptor:Prothrombin, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
Authors:Pineda, A., Chen, Z., Mathews, F.S., Di Cera, E.
Deposit date:2006-04-17
Release date:2006-09-12
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.87 Å)
Cite:Crystal structure of thrombin in a self-inhibited conformation.
J.Biol.Chem., 281, 2006
2OCV
  • Download 2ocv
  • View 2ocv
Molmil generated image of 2ocv
STRUCTURAL BASIS OF NA+ ACTIVATION MIMICRY IN MURINE THROMBIN
Descriptor:Thrombin, N-ACETYL-D-GLUCOSAMINE
Authors:Marino, F., Chen, Z., Ergenekan, C.E., Bush, L.A., Mathews, F.S., Di Cera, E.
Deposit date:2006-12-21
Release date:2007-04-10
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Structural basis of na+ activation mimicry in murine thrombin.
J.Biol.Chem., 282, 2007
2OD3
  • Download 2od3
  • View 2od3
Molmil generated image of 2od3
HUMAN THROMBIN CHIMERA WITH HUMAN RESIDUES 184A, 186, 186A, 186B, 186C AND 222 REPLACED BY MURINE THROMBIN EQUIVALENTS.
Descriptor:Thrombin light chain, Thrombin heavy chain, D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide, ...
Authors:Marino, F., Chen, Z., Ergenekan, C.E., Bush, L.A., Mathews, F.S., Di Cera, E.
Deposit date:2006-12-21
Release date:2007-04-10
Last modified:2013-02-27
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Structural basis of na+ activation mimicry in murine thrombin.
J.Biol.Chem., 282, 2007
2PGB
  • Download 2pgb
  • View 2pgb
Molmil generated image of 2pgb
INHIBITOR-FREE HUMAN THROMBIN MUTANT C191A-C220A
Descriptor:Prothrombin, N-ACETYL-D-GLUCOSAMINE, SULFATE ION
Authors:Bush-Pelc, L.A., Marino, F., Chen, Z., Pineda, A.O., Mathews, F.S., Di Cera, E.
Deposit date:2007-04-09
Release date:2007-07-17
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.54 Å)
Cite:Important role of the cys-191 cys-220 disulfide bond in thrombin function and allostery
J.Biol.Chem., 282, 2007
2PGQ
  • Download 2pgq
  • View 2pgq
Molmil generated image of 2pgq
HUMAN THROMBIN MUTANT C191A-C220A IN COMPLEX WITH THE INHIBITOR PPACK
Descriptor:Prothrombin, ZINC ION, D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide, ...
Authors:Bush-Pelc, L.A., Marino, F., Chen, Z., Pineda, A.O., Mathews, F.S., Di Cera, E.
Deposit date:2007-04-10
Release date:2007-07-17
Last modified:2013-09-25
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Important role of the cys-191 cys-220 disulfide bond in thrombin function and allostery
J.Biol.Chem., 282, 2007
3BEI
  • Download 3bei
  • View 3bei
Molmil generated image of 3bei
CRYSTAL STRUCTURE OF THE SLOW FORM OF THROMBIN IN A SELF_INHIBITED CONFORMATION
Descriptor:Prothrombin, N-ACETYL-D-GLUCOSAMINE, GLYCEROL
Authors:Gandhi, P.S., Chen, Z., Mathews, F.S., Di Cera, E.
Deposit date:2007-11-19
Release date:2007-12-25
Last modified:2018-01-24
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:Structural identification of the pathway of long-range communication in an allosteric enzyme.
Proc.Natl.Acad.Sci.Usa, 105, 2008
3BV9
  • Download 3bv9
  • View 3bv9
Molmil generated image of 3bv9
STRUCTURE OF THROMBIN BOUND TO THE INHIBITOR FM19
Descriptor:Thrombin light chain, Thrombin heavy chain, FM19 inhibitor, ...
Authors:Nieman, M.T., Burke, F., Warnock, M., Zhou, Y., Sweigert, J., Chen, A., Ricketts, D., Lucchesi, B.R., Chen, Z., Di Cera, E., Hilfinger, J., Mosberg, H.I., Schmaier, A.H.
Deposit date:2008-01-05
Release date:2008-03-04
Last modified:2012-12-12
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Thrombostatin FM compounds: direct thrombin inhibitors - mechanism of action in vitro and in vivo.
J.Thromb.Haemost., 6, 2008
3EDX
  • Download 3edx
  • View 3edx
Molmil generated image of 3edx
CRYSTAL STRUCTURE OF THE W215A/E217A MUTANT OF MURINE THROMBIN
Descriptor:Thrombin light chain, Thrombin heavy chain, SULFATE ION, ...
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-pelc, L., Di Cera, E.
Deposit date:2008-09-03
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Molecular Basis for the Kinetic Differences of the W215A/E217A Mutant of Human and Murine Thrombin
To be Published
3EE0
  • Download 3ee0
  • View 3ee0
Molmil generated image of 3ee0
CRYSTAL STRUCTURE OF THE W215A/E217A MUTANT OF HUMAN THROMBIN (SPACE GROUP P2(1)2(1)2(1))
Descriptor:Thrombin light chain, Thrombin heavy chain
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-Pelc, L., Di Cera, E.
Deposit date:2008-09-03
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.75 Å)
Cite:Molecular Basis for the Kinetic Differences of the W215A/E217A Mutant of Human and Murine Thrombin
To be Published
3HK3
  • Download 3hk3
  • View 3hk3
Molmil generated image of 3hk3
CRYSTAL STRUCTURE OF MURINE THROMBIN MUTANT W215A/E217A (ONE MOLECULE IN THE ASYMMETRIC UNIT)
Descriptor:Thrombin light chain, Thrombin heavy chain
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-Pelc, L., Di Cera, E.
Deposit date:2009-05-22
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A.
J.Biol.Chem., 284, 2009
3HK6
  • Download 3hk6
  • View 3hk6
Molmil generated image of 3hk6
CRYSTAL STRUCTURE OF MURINE THROMBIN MUTANT W215A/E217A (TWO MOLECULES IN THE ASYMMETRIC UNIT)
Descriptor:Thrombin light chain, Thrombin heavy chain
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-Pelc, L., Di Cera, E.
Deposit date:2009-05-22
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A.
J.Biol.Chem., 284, 2009
3HKI
  • Download 3hki
  • View 3hki
Molmil generated image of 3hki
CRYSTAL STRUCTURE OF MURINE THROMBIN MUTANT W215A/E217A IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF HUMAN PAR1
Descriptor:Thrombin light chain, Thrombin heavy chain, Proteinase-activated receptor 1, ...
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-Pelc, L., Di Cera, E.
Deposit date:2009-05-23
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A.
J.Biol.Chem., 284, 2009
3HKJ
  • Download 3hkj
  • View 3hkj
Molmil generated image of 3hkj
CRYSTAL STRUCTURE OF HUMAN THROMBIN MUTANT W215A/E217A IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF HUMAN PAR1
Descriptor:Thrombin light chain, Thrombin heavy chain, Proteinase-activated receptor 1, ...
Authors:Gandhi, P.S., Page, M.J., Chen, Z., Bush-Pelc, L., Di Cera, E.
Deposit date:2009-05-23
Release date:2009-07-07
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A.
J.Biol.Chem., 284, 2009
3LU9
  • Download 3lu9
  • View 3lu9
Molmil generated image of 3lu9
CRYSTAL STRUCTURE OF HUMAN THROMBIN MUTANT S195A IN COMPLEX WITH THE EXTRACELLULAR FRAGMENT OF HUMAN PAR1
Descriptor:Prothrombin, Proteinase-activated receptor 1, N-ACETYL-D-GLUCOSAMINE, ...
Authors:Gandhi, P.S., Chen, Z., Di Cera, E.
Deposit date:2010-02-17
Release date:2010-03-16
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Crystal structure of thrombin bound to the uncleaved extracellular fragment of PAR1.
J.Biol.Chem., 285, 2010
3NXP
  • Download 3nxp
  • View 3nxp
Molmil generated image of 3nxp
CRYSTAL STRUCTURE OF HUMAN PRETHROMBIN-1
Descriptor:Prethrombin-1, N-ACETYL-D-GLUCOSAMINE, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ...
Authors:Chen, Z., Bush-Pelc, L.A., Di Cera, E.
Deposit date:2010-07-14
Release date:2010-11-10
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystal structure of prethrombin-1.
Proc.Natl.Acad.Sci.USA, 107, 2010
3QGN
  • Download 3qgn
  • View 3qgn
Molmil generated image of 3qgn
THE ALLOSTERIC E*-E EQUILIBRIUM IS A KEY PROPERTY OF THE TRYPSIN FOLD
Descriptor:Thrombin light chain, Thrombin heavy chain, IODIDE ION, ...
Authors:Niu, W., Gohara, D., Chen, Z., Di Cera, E.
Deposit date:2011-01-24
Release date:2011-07-06
Last modified:2017-11-08
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystallographic and kinetic evidence of allostery in a trypsin-like protease.
Biochemistry, 50, 2011
3R3G
  • Download 3r3g
  • View 3r3g
Molmil generated image of 3r3g
STRUCTURE OF HUMAN THROMBIN WITH RESIDUES 145-150 OF MURINE THROMBIN.
Descriptor:Thrombin Light Chain, Thrombin Heavy Chain, N-ACETYL-D-GLUCOSAMINE, ...
Authors:Pozzi, N., Chen, R., Chen, Z., Bah, A., Di Cera, E.
Deposit date:2011-03-15
Release date:2011-05-11
Last modified:2011-09-21
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Rigidification of the autolysis loop enhances Na(+) binding to thrombin.
Biophys.Chem., 159, 2011