4A93

RNA Polymerase II elongation complex containing a CPD Lesion

Summary for 4A93

• Entry DOI: 10.2210/pdb4a93/pdb
• Related: 1A1D 1DZF 1I3Q 1I50 1I6H 1K83 1NIK 1NT9 1PQV 1R5U 1R9S 1R9T 1SFO 1TWA 1TWC 1TWF 1TWG 1TWH 1WCM 1Y14 1Y1V 1Y1W 1Y1Y 1Y77 2B63 2B8K 2JA5 2JA6 2JA7 2JA8 2VUM 4A3B 4A3C 4A3D 4A3E 4A3F 4A3G 4A3I 4A3J 4A3K 4A3L 4A3M
• Descriptor: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5, DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11, ... (17 entities in total)
• Functional Keywords: transcription, transcription fidelity, transcription coupled dna repair, dna damage, dna repair, pyrimidine dimers
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Total number of polymer chains: 15
• Total formula weight: 530612.81

Authors

Walmacq, C.,Cheung, A.C.M.,Kireeva, M.L.,Lubkowska, L.,Ye, C.,Gotte, D.,Strathern, J.N.,Carell, T.,Cramer, P.,Kashlev, M. (deposition date: 2011-11-23, release date: 2012-03-21, Last modification date: 2024-10-16)

Primary citation

Walmacq, C.,Cheung, A.C.,Kireeva, M.L.,Lubkowska, L.,Ye, C.,Gotte, D.,Strathern, J.N.,Carell, T.,Cramer, P.,Kashlev, M.
Mechanism of Translesion Transcription by RNA Polymerase II and its Role in Cellular Resistance to DNA Damage.
Mol.Cell, 46:18-, 2012

PubMed Abstract: UV-induced cyclobutane pyrimidine dimers (CPDs) in the template DNA strand stall transcription elongation by RNA polymerase II (Pol II). If the nucleotide excision repair machinery does not promptly remove the CPDs, stalled Pol II creates a roadblock for DNA replication and subsequent rounds of transcription. Here we present evidence that Pol II has an intrinsic capacity for translesion synthesis (TLS) that enables bypass of the CPD with or without repair. Translesion synthesis depends on the trigger loop and bridge helix, the two flexible regions of the Pol II subunit Rpb1 that participate in substrate binding, catalysis, and translocation. Substitutions in Rpb1 that promote lesion bypass in vitro increase UV resistance in vivo, and substitutions that inhibit lesion bypass decrease cell survival after UV irradiation. Thus, translesion transcription becomes essential for cell survival upon accumulation of the unrepaired CPD lesions in genomic DNA.

PubMed: 22405652
DOI: 10.1016/J.MOLCEL.2012.02.006

Experimental method

X-RAY DIFFRACTION (3.4 Å)