1I6H

RNA POLYMERASE II ELONGATION COMPLEX

Summary for 1I6H

• Entry DOI: 10.2210/pdb1i6h/pdb
• Related: 1I3Q 1I50
• Descriptor: 5'-D(P*AP*AP*AP*TP*GP*CP*CP*TP*GP*GP*TP*CP*T)-3', DNA-DIRECTED RNA POLYMERASE II 8.3KD POLYPEPTIDE, DNA-DIRECTED RNA POLYMERASE II 13.6KD POLYPEPTIDE, ... (14 entities in total)
• Functional Keywords: transcription, mrna, multiprotein complex, molecular machine, dna, transcription-dna-rna complex, transcription-dna-rna hybrid complex, transcription/dna-rna hybrid
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Nucleus: P04050 P08518 P16370 P20434 P20436 P38902; Nucleus, nucleolus : P40422 P27999 P22139; Cytoplasm : P20435
• Total number of polymer chains: 12
• Total formula weight: 477033.97

Authors

Gnatt, A.L.,Cramer, P.,Kornberg, R.D. (deposition date: 2001-03-02, release date: 2001-04-23, Last modification date: 2023-08-09)

Primary citation

Gnatt, A.L.,Cramer, P.,Fu, J.,Bushnell, D.A.,Kornberg, R.D.
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.
Science, 292:1876-1882, 2001

PubMed Abstract: The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 A resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3' end of the RNA in the nucleotide addition site. The 3' end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back-tracking. The 5'-most residue of the RNA is close to the point of entry to an exit groove. Changes in protein structure between the transcribing complex and free enzyme include closure of a clamp over the DNA and RNA and ordering of a series of "switches" at the base of the clamp to create a binding site complementary to the DNA-RNA hybrid. Protein-nucleic acid contacts help explain DNA and RNA strand separation, the specificity of RNA synthesis, "abortive cycling" during transcription initiation, and RNA and DNA translocation during transcription elongation.

PubMed: 11313499
DOI: 10.1126/science.1059495

Experimental method

X-RAY DIFFRACTION (3.3 Å)