2XCP

TERNARY COMPLEX OF SULFOLOBUS SOLFATARICUS DPO4 DNA POLYMERASE, 7,8- DIHYDRO-8-OXODEOXYGUANINE MODIFIED DNA AND dCTP - MAGNESIUM FORM

Summary for 2XCP

• Entry DOI: 10.2210/pdb2xcp/pdb
• Related: 1JX4 1JXL 1N48 1N56 1RYR 1RYS 1S0M 1S0N 1S0O 1S10 1S97 1S9F 2AGO 2AGP 2AGQ 2ASD 2ASJ 2ASL 2ATL 2AU0 2BQ3 2BQR 2BQU 2BR0 2C22 2C28 2C2D 2C2E 2C2R 2J6S 2J6T 2J6U 2JEF 2JEG 2JEI 2JEJ 2UVR 2UVU 2UVV 2UVW 2V4Q 2V4R 2V4S 2V4T 2V9W 2VA2 2VA3 2W8K 2W8L 2W9A 2W9B 2W9C 2XC9 2XCA
• Descriptor: DNA POLYMERASE IV, 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*TP *TP*DOC)-3', 5'-D(*TP*CP*AP*CP*8OGP*GP*AP*AP*TP*CP*CP *TP*TP*CP*CP*CP*CP*C)-3', ... (6 entities in total)
• Functional Keywords: dna-transferase complex, translesion dna polymerase, nucleotidyltransferase, transferase, dna/transferase
• Biological source: SULFOLOBUS SOLFATARICUS
• Cellular location: Cytoplasm (Probable): Q97W02
• Total number of polymer chains: 6
• Total formula weight: 102191.95

Authors

Irimia, A.,Egli, M. (deposition date: 2010-04-24, release date: 2010-09-08, Last modification date: 2023-12-20)

Primary citation

Irimia, A.,Loukachevitch, L.V.,Eoff, R.L.,Guengerich, F.P.,Egli, M.
Metal Ion Dependence of the Active Site Conformation of the Trans-Lesion DNA Polymerase Dpo4 from Sulfolobus Solfataricus
Acta Crystallogr.,Sect.F, 66:1013-, 2010

PubMed Abstract: Crystal structures of a binary Mg2+-form Dpo4-DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4-DNA-dCTP/dGTP complexes with 8-oxoG in the template strand have been determined. Comparison of their conformations and active-site geometries with those of the corresponding Ca2+-form complexes revealed that the DNA and polymerase undergo subtle changes as a result of the catalytically more active Mg2+ occupying both the A and B sites.

PubMed: 20823515
DOI: 10.1107/S1744309110029374

Experimental method

X-RAY DIFFRACTION (2.6 Å)