1CWA

X-RAY STRUCTURE OF A MONOMERIC CYCLOPHILIN A-CYCLOSPORIN A CRYSTAL COMPLEX AT 2.1 ANGSTROMS RESOLUTION

1CWA の概要

• エントリーDOI: 10.2210/pdb1cwa/pdb
• 関連するPDBエントリー: 1BCK 1C5F 1CSA 1CWB 1CWC 1CWF 1CWH 1CWI 1CWJ 1CWK 1CWL 1CWM 1CWO 1CYA 1CYB 1CYN 1IKF 1M63 1MF8 1MIK 1QNG 1QNH 1XQ7 2ESL 2OJU 2POY 2RMA 2RMB 2RMC 2WFJ 2X2C 2X7K 2Z6W 3BO7 3CYS 3EOV
• 関連するBIRD辞書のPRD_ID: PRD_000142
• 分子名称: PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A, CYCLOSPORIN A (3 entities in total)
• 機能のキーワード: isomerase-immunosuppressant complex, cyclophilin-cyclosporin complex, cyclosporin a, immunosuppressant, cyclophilin, isomerase/immunosuppressant
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19257.13

構造登録者

Mikol, V.,Kallen, J.,Walkinshaw, M.D. (登録日: 1995-09-06, 公開日: 1996-01-29, 最終更新日: 2024-06-05)

主引用文献

Mikol, V.,Kallen, J.,Pflugl, G.,Walkinshaw, M.D.
X-Ray Structure of a Monomeric Cyclophilin A-Cyclosporin a Crystal Complex at 2.1 A Resolution.
J.Mol.Biol., 234:1119-, 1993

PubMed Abstract: The crystal structure of a complex between recombinant human cyclophilin A (Cyp) and cyclosporin A (CsA) has been determined from a novel orthorhombic crystal form that contains only one monomer of complex per asymmetric unit rather than five in the previously determined tetragonal structure. The structure has been refined at 2.1 A resolution to a crystallographic R-factor of 16.7%. The conformation of Cyp is practically unchanged with respect to the tetragonal form. A certain number of previously undefined side-chains have been located in the electron density and a very detailed picture of the ordered solvent structure has been obtained. The interactions between CsA and Cyp are conserved. A network of the possibly conserved, water-mediated contacts is described. The structure of CsA in the monomeric complex is similar to that of the decameric complex, but shows a few small differences in the so-called effector domain of CsA, probably due to differences in crystal environment. The fact that this monomeric crystal form can be obtained shows that the formation of pentamer or decamer complexes is not a generally observed phenomenon and is not a prerequisite for biological activity.

PubMed: 8263916
DOI: 10.1006/JMBI.1993.1664

実験手法

X-RAY DIFFRACTION (2.1 Å)