PDB-4z3o: Quinolone(Moxifloxacin)-DNA cleavage complex of topoisomerase IV ...

ID or keywords:

Entry

Database: PDB / ID: 4z3o

Title

Quinolone(Moxifloxacin)-DNA cleavage complex of topoisomerase IV from S. pneumoniae

Components

(E-site DNA) x 4
DNA topoisomerase 4 subunit B,ParE30-ParC55 fused topo IV from S. pneumoniae

Keywords

ISOMERASE / Topo IV / Cleavage complex / DNA / quinolone

Function / homology

Function and homology information

DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex / DNA negative supercoiling activity / DNA topoisomerase (ATP-hydrolysing) / extrinsic component of plasma membrane / DNA topological change / chromosome segregation / chromosome / DNA binding / ATP binding / metal ion binding / cytoplasm
Similarity search - Function

Biological species

Streptococcus pneumoniae (bacteria)

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.44 Å

Authors

Laponogov, I. / Veselkov, D.A. / Pan, X.-S. / Selvarajah, J. / Crevel, I.M.-T. / Fisher, L.M. / Sanderson, M.R.

Funding support

United Kingdom, 1items

Citation

Journal: To Be Published
Title: Structural studies of the drug-stabilized cleavage complexes of topoisomerase IV and gyrase from Streptococcus pneumoniae
Authors: Laponogov, I. / Veselkov, D.A. / Pan, X.-S. / Selvarajah, J. / Crevel, I.M.-T. / Fisher, L.M. / Sanderson, M.R.

History

Deposition	Mar 31, 2015	Deposition site: RCSB / Processing site: PDBE
Revision 1.0	Sep 14, 2016	Provider: repository / Type: Initial release
Revision 1.1	Aug 30, 2017	Group: Author supporting evidence / Derived calculations / Category: pdbx_audit_support / struct_conn / Item: _pdbx_audit_support.funding_organization
Revision 1.2	Jan 10, 2024	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_conn_type Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn_type.id
Revision 1.3	Oct 23, 2024	Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

Summary document	4z3o_validation.pdf.gz	1001.5 KB	Display	wwPDB validaton report
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Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/z3/4z3o ftp://data.pdbj.org/pub/pdb/validation_reports/z3/4z3o	HTTPS FTP

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Related structure data

Related structure data	4z2cC 4z2dC 4z2eC 4z4qC 4z53C 3k9fS 3ksa 3ksb 3rad 3rae 3raf 4i3h 4koe 4pxp PDB Unreleased entry S: Starting model for refinement C: citing same article (ref.)
Similar structure data	4kpe-d 4kpf-d 3raf-d 3rad-d 4bul-d 4z4q-d 5iwm-d 3ltn-d 5cdq-2 6waa-1 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
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PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: DNA topoisomerase 4 subunit B,ParE30-ParC55 fused topo IV from S. pneumoniae

B: DNA topoisomerase 4 subunit B,ParE30-ParC55 fused topo IV from S. pneumoniae

E: E-site DNA

F: E-site DNA

G: E-site DNA

H: E-site DNA

hetero molecules

180 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by software

Unit cell

Length a, b, c (Å)	157.760, 157.760, 211.020
Angle α, β, γ (deg.)	90.00, 90.00, 120.00
Int Tables number	152
Space group name H-M	P3₁21

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Protein , 1 types, 2 molecules AB

#1: Protein	DNA topoisomerase 4 subunit B,ParE30-ParC55 fused topo IV from S. pneumoniae / Topoisomerase IV subunit B Mass: 84211.422 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae (bacteria) / Gene: parE, SP_0852, Z67739 / Production host: Escherichia coli (E. coli) / References: UniProt: Q59961, UniProt: P72525, EC: 5.99.1.3

#1: Protein

DNA topoisomerase 4 subunit B,ParE30-ParC55 fused topo IV from S. pneumoniae / Topoisomerase IV subunit B

Mass: 84211.422 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Streptococcus pneumoniae (bacteria) / Gene: parE, SP_0852, Z67739 / Production host:

Escherichia coli (E. coli) / References: UniProt: Q59961, UniProt: P72525, EC: 5.99.1.3

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DNA chain , 4 types, 4 molecules EFGH

#2: DNA chain	E-site DNA Mass: 2121.436 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#3: DNA chain	E-site DNA Mass: 3348.209 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#4: DNA chain	E-site DNA Mass: 2113.410 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#5: DNA chain	E-site DNA Mass: 3358.211 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)

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Non-polymers , 3 types, 20 molecules

#6: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#7: Chemical	ChemComp-MFX / 1-cyclopropyl-6-fluoro-8-methoxy-7-[(4aS,7aS)-octahydro-6H-pyrrolo[3,4-b]pyridin-6-yl]-4-oxo-1,4-dihydroquinoline-3-carboxylic acid / moxifloxacin Mass: 401.431 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₂₁H₂₄FN₃O₄
#8: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 14 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has protein modification	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION

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Sample preparation

Crystal	Density Matthews: 4.85 Å³/Da / Density % sol: 74.66 %
Crystal grow	Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 50 mM Na Cacodylate, 100-150 mM NaCl, 4-7% isopropanol PH range: 6.0-7.0 / Temp details: Incubator

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
Detector	Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 4, 2012
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9763 Å / Relative weight: 1
Reflection	Resolution: 3.17→70.34 Å / Num. obs: 52030 / % possible obs: 99.94 % / Redundancy: 10.1 % / R_merge(I) obs: 0.093 / Net I/σ(I): 5.9986
Reflection shell	Resolution: 3.17→3.25 Å / Redundancy: 9.76 % / R_merge(I) obs: 0.95 / Mean I/σ(I) obs: 0.8 / % possible all: 100

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Processing

Software

Name	Version	Classification
PHENIX	1.8_1069	refinement
xia2		data reduction
xia2		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 3K9F

3k9f

Resolution: 3.44→70.34 Å / SU ML: 0.33 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 22 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.212	1984	4.86 %	Random
R_work	0.1819	-	-	-
obs	0.1834	40861	99.89 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.44→70.34 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	10247	730	62	14	11053

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	11308
X-RAY DIFFRACTION	f_angle_d	0.632	15529
X-RAY DIFFRACTION	f_dihedral_angle_d	14.331	4110
X-RAY DIFFRACTION	f_chiral_restr	0.042	1784
X-RAY DIFFRACTION	f_plane_restr	0.002	1902

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.4401-3.5261	0.2775	147	0.2322	2739	X-RAY DIFFRACTION	100
3.5261-3.6214	0.256	146	0.2152	2715	X-RAY DIFFRACTION	100
3.6214-3.728	0.2413	103	0.1951	2781	X-RAY DIFFRACTION	100
3.728-3.8483	0.2633	141	0.1898	2737	X-RAY DIFFRACTION	100
3.8483-3.9858	0.2504	132	0.1784	2755	X-RAY DIFFRACTION	100
3.9858-4.1454	0.1983	146	0.1739	2758	X-RAY DIFFRACTION	100
4.1454-4.3341	0.1946	147	0.1608	2745	X-RAY DIFFRACTION	100
4.3341-4.5625	0.1987	136	0.1481	2755	X-RAY DIFFRACTION	100
4.5625-4.8483	0.2052	143	0.1522	2764	X-RAY DIFFRACTION	100
4.8483-5.2225	0.2175	122	0.167	2798	X-RAY DIFFRACTION	100
5.2225-5.7479	0.2389	136	0.1859	2799	X-RAY DIFFRACTION	100
5.7479-6.5791	0.2499	145	0.207	2812	X-RAY DIFFRACTION	100
6.5791-8.2869	0.2052	167	0.1845	2800	X-RAY DIFFRACTION	100
8.2869-70.3547	0.1723	173	0.1911	2919	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.153	-0.1015	0.165	9.3462	-7.0884	5.4209	0.1009	0.0671	-0.0113	-0.217	-0.3926	-0.5667	-0.2371	0.6345	0.1649	0.5631	-0.0151	0.0652	0.5015	-0.1195	0.4339	78.5233	-39.6093	-9.2058
2	6.116	0.06	-0.1651	8.2441	1.1959	8.9858	0.1575	0.575	-0.2477	-0.4117	-0.3129	-1.3034	0.1874	1.0032	0.1491	0.3039	0.0144	0.0288	0.5701	0.1072	0.5004	85.3246	-61.8652	8.0424
3	3.1132	-2.2141	3.1328	3.3441	0.6773	7.8054	0.2905	-2.0656	1.0152	-0.5226	0.5443	0.3175	0.3466	0.5556	-0.645	0.9718	-0.4859	0.2296	1.1056	-0.3321	0.8013	89.4378	-7.1209	-4.2192
4	0.0211	-0.0072	0.0656	2.2901	-1.3308	0.9123	-0.2657	-0.4832	-0.8249	-0.3649	-0.5725	-1.3186	0.1283	0.9661	0.7923	0.4212	-0.5653	0.8722	1.6903	-0.3401	1.2089	105.9751	1.2576	-0.7664
5	2.5152	-0.8899	-0.133	4.0925	1.5517	3.8348	-0.0165	-0.3206	0.2104	0.2681	0.1257	0.0996	-0.1951	0.0705	-0.0986	0.8357	-0.0716	0.0706	0.5614	0.0007	0.4363	73.2402	-15.863	3.8921
6	2.9823	1.7053	-0.191	5.8708	-5.6525	7.8609	0.0518	-0.4898	0.1016	-0.5181	0.4964	0.3453	0.8585	-0.4035	-0.224	0.7231	0.176	0.0922	0.6943	-0.122	0.466	72.7574	-51.669	-9.1939
7	5.011	-1.921	-0.1006	5.7567	1.7264	2.2443	-0.2292	-0.246	0.0565	0.1555	0.2326	0.813	-0.878	-0.408	-0.0359	1.1658	0.286	0.0242	0.6	0.1649	0.6633	53.3071	12.5044	-8.6404
8	5.6259	-5.0312	-1.605	9.601	2.0708	1.9664	-0.1526	-0.4581	-0.1199	0.1526	0.1639	0.0595	0.2363	-0.0479	0.0109	0.6917	-0.1471	0.0312	0.4215	-0.0192	0.2819	64.7394	-24.5084	-16.9412
9	2.9199	-0.8456	-0.2546	2.4084	-1.4273	2.9692	0.1297	0.1157	0.433	-0.2653	0.1169	0.0559	-0.5951	0.2323	-0.2628	0.9193	-0.1904	0.1473	0.459	-0.051	0.4807	71.7335	-9.5403	-18.4798
10	-0.0495	0.1652	-0.1407	3.5631	2.2745	2.0176	0.1495	0.2845	-0.0869	-0.0471	-0.1151	0.0796	-0.1889	-0.3661	0.0973	0.3414	0.0079	-0.0689	0.6194	0.1335	0.3636	77.2052	-41.8149	40.9054
11	4.7775	-0.1008	-1.2047	7.9653	-0.9426	7.7009	0.0657	-0.2646	-0.0932	-0.3178	0.1787	0.5849	0.6272	-0.6173	-0.1649	0.3255	-0.1309	-0.0593	0.5481	0.0212	0.2982	74.103	-63.81	21.5636
12	6.604	0.8706	5.2225	2.0773	1.7173	4.672	0.2458	1.3578	0.4231	0.0267	0.4437	-0.6382	-0.4157	-1.0884	-0.5076	0.7002	0.0337	0.2111	1.0263	0.3814	0.6731	63.7358	-9.4988	38.5607
13	1.047	1.0482	0.5837	1.0276	0.5995	0.3354	0.4585	-0.2965	-1.0784	0.9306	0.0558	1.2192	-0.0654	-0.9885	-0.2195	0.4113	0.0517	0.057	1.0499	0.3673	1.1747	46.9966	-2.3482	36.2684
14	3.2779	1.1303	-0.0675	5.2659	-0.1132	4.7934	-0.109	0.0972	0.187	-0.4791	0.258	0.1579	0.3943	-0.1439	-0.1156	0.6041	-0.0633	-0.055	0.4593	0.0732	0.3315	80.2619	-16.7128	29.625
15	5.1301	-3.1644	-1.6483	5.1159	6.2378	9.2038	0.1161	0.0378	0.1186	0.491	0.6767	-0.8093	0.1269	0.3491	-0.5937	0.7741	-0.1468	0.0235	0.4772	0.1303	0.4544	84.1371	-52.7043	39.8568
16	7.9303	1.6086	0.8617	7.1542	-0.9648	4.4721	0.2268	0.2484	0.0846	-0.1977	-0.0317	-0.593	-0.2747	0.5143	-0.16	0.6569	-0.1336	-0.005	0.4693	-0.147	0.514	99.3443	11.1952	44.3678
17	2.9202	2.3998	-0.1902	6.3012	-1.7553	0.8912	0.0482	-0.1529	0.0761	0.1399	-0.163	-0.4134	0.3671	0.0289	0.1391	0.7193	0.1066	-0.0113	0.5725	0.0172	0.2821	89.4239	-26.1347	49.4007
18	1.9661	-0.1335	-0.2911	3.1009	0.5145	1.2501	0.1254	-0.4481	0.1359	0.6152	0.1587	-0.1142	-0.0216	-0.0781	-0.3012	0.7672	0.0128	-0.0148	0.555	0.036	0.3543	81.5519	-12.2865	52.3301
19	1.0477	0.207	0.7996	6.1012	-5.8815	9.4134	-0.6763	0.0276	-1.84	-0.7681	0.8734	0.0032	1.0488	1.316	-0.1519	0.5361	0.0043	0.1936	1.0209	-0.3258	1.1786	104.4264	-15.1022	12.2941
20	6.3089	0.0726	-2.218	6.4673	-1.9923	2.6188	-0.3981	0.8897	1.1233	-1.0065	0.79	-0.3703	-0.5991	0.6728	-0.3263	1.3185	-0.6132	0.3903	1.5351	-0.0636	1.0366	97.6238	5.7569	-11.7209
21	3.2112	-0.3568	0.0037	-0.0696	-0.3335	1.4107	-0.6018	-0.1711	0.6386	-0.2351	0.4395	-0.4775	-0.4616	0.5264	0.1779	1.2015	-0.5109	0.0576	1.2391	-0.2761	1.1038	100.5046	7.7441	10.8198
22	0.3084	-1.3767	-0.9836	6.8728	5.8082	5.7985	-0.0283	1.3223	-0.0512	-0.2051	0.7159	1.2374	-0.3709	-0.0433	-0.6019	0.5893	-0.071	-0.0509	0.9001	0.0298	1.2238	49.2607	-17.0751	20.8123
23	1.4774	-0.8023	0.6099	1.3806	0.6231	1.1709	0.3655	-0.5811	0.548	0.6641	-0.3024	1.3036	-0.5916	-1.054	0.0478	1.1238	0.5062	0.3073	1.2955	0.2928	1.2642	54.3518	2.2259	46.8381
24	2.2109	1.122	0.0059	1.408	0.0789	1.391	-0.3714	0.238	0.5675	-0.2055	0.42	0.9305	-0.4934	-0.2722	-0.0079	0.8347	0.313	-0.0132	1.0458	0.344	1.1259	51.9251	5.8375	24.8089
25	8.7071	-0.6107	-0.5628	8.0987	-2.7898	1.0133	0.2592	-0.2848	1.0329	-0.676	0.7468	-0.3602	-1.3723	0.7039	-1.0465	1.2867	0.0058	0.283	1.2081	-0.1385	0.7542	73.8157	3.475	33.5981
26	2.4701	-0.1959	-1.7698	6.8449	4.068	3.5968	0.1953	-0.4429	0.9233	0.795	0.5132	0.5151	-1.3722	-0.2803	-0.8023	1.7336	-0.2241	0.1686	1.2403	0.038	0.8777	78.3598	4.8989	1.7466
27	5.3522	2.4837	-1.3737	6.1251	-2.0044	0.728	0.5559	0.3294	-0.3514	0.3662	-2.2568	-1.3131	0.7988	-0.816	1.4506	1.6092	-0.147	0.4668	1.1534	0.1957	0.9215	81.6047	4.5595	11.1827
28	7.7344	-2.8512	0.4843	1.3466	0.6652	2.4375	0.3673	-0.0436	-0.3069	0.0499	-0.3829	-0.0121	1.0563	0.2508	0.1113	1.1699	0.1218	0.3871	1.0587	-0.008	0.6614	70.485	3.7465	24.0856

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain "A" and resseq 1343:1382
2	X-RAY DIFFRACTION	2	chain "A" and resseq 1383:1429
3	X-RAY DIFFRACTION	3	chain "A" and resseq 1018:1030
4	X-RAY DIFFRACTION	4	chain "A" and resseq 1002:1017
5	X-RAY DIFFRACTION	5	chain "A" and resseq 1031:1154
6	X-RAY DIFFRACTION	6	chain "A" and resseq 1430:1455
7	X-RAY DIFFRACTION	7	chain "A" and resseq 1239:1322
8	X-RAY DIFFRACTION	8	chain "A" and resseq 1456:1482
9	X-RAY DIFFRACTION	9	chain "A" and (resseq 1155:1238 or resseq 1323:1342)
10	X-RAY DIFFRACTION	10	chain "B" and resseq 1343:1382
11	X-RAY DIFFRACTION	11	chain "B" and resseq 1383:1429
12	X-RAY DIFFRACTION	12	chain "B" and resseq 1018:1030
13	X-RAY DIFFRACTION	13	chain "B" and resseq 1002:1017
14	X-RAY DIFFRACTION	14	chain "B" and resseq 1031:1154
15	X-RAY DIFFRACTION	15	chain "B" and resseq 1430:1455
16	X-RAY DIFFRACTION	16	chain "B" and resseq 1239:1322
17	X-RAY DIFFRACTION	17	chain "B" and resseq 1456:1482
18	X-RAY DIFFRACTION	18	chain "B" and (resseq 1155:1238 or resseq 1323:1342)
19	X-RAY DIFFRACTION	19	chain "A" and resseq 539:581
20	X-RAY DIFFRACTION	20	chain "A" and resseq 610:634
21	X-RAY DIFFRACTION	21	chain "A" and (resseq 415:538 or resseq 582:609 or resseq 635:640 or resid 742)
22	X-RAY DIFFRACTION	22	chain "B" and resseq 539:581
23	X-RAY DIFFRACTION	23	chain "B" and resseq 610:634
24	X-RAY DIFFRACTION	24	chain "B" and (resseq 415:538 or resseq 582:609 or resseq 635:640 or resid 742)
25	X-RAY DIFFRACTION	25	(chain "E" or chain "F" ) and resseq 1:20
26	X-RAY DIFFRACTION	26	(chain "G" or chain "H" ) and resseq 1:20
27	X-RAY DIFFRACTION	27	(chain "E" or chain "F" ) and resseq 101:102
28	X-RAY DIFFRACTION	28	(chain "G" or chain "H" ) and resseq 101:102

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