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- PDB-4i3h: A three-gate structure of topoisomerase IV from Streptococcus pne... -

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Basic information

Entry
Database: PDB / ID: 4i3h
TitleA three-gate structure of topoisomerase IV from Streptococcus pneumoniae
Components
  • DNA (5'-D(*CP*AP*AP*AP*GP*GP*CP*GP*GP*TP*AP*AP*TP*AP*CP*GP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*GP*AP*AP*TP*CP*AP*GP*G)-3')
  • DNA (5'-D(*CP*CP*TP*GP*AP*TP*TP*CP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*CP*GP*TP*AP*TP*TP*AP*CP*CP*GP*CP*CP*TP*TP*TP*G)-3')
  • Topoisomerase IV subunit B, DNA topoisomerase 4 subunit A chimera
KeywordsISOMERASE/DNA / DNA unwinding / supercoiling / ISOMERASE-DNA complex
Function / homology
Function and homology information


DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity / DNA topoisomerase (ATP-hydrolysing) / extrinsic component of plasma membrane / DNA topological change / chromosome segregation / chromosome / DNA binding / ATP binding / metal ion binding
Similarity search - Function
DNA topoisomerase IV subunit A, Gram-positive / DNA topoisomerase 4 subunit B, Firmicutes/Mollicutes / Rossmann fold - #670 / DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat / DNA gyrase/topoisomerase IV, subunit A, C-terminal / DNA gyrase C-terminal domain, beta-propeller / DNA topoisomerase, type IIA, subunit B / DNA topoisomerase, type IIA, alpha-helical domain superfamily / DNA topoisomerase, type IIA, domain A / DNA topoisomerase, type IIA, domain A, alpha-beta ...DNA topoisomerase IV subunit A, Gram-positive / DNA topoisomerase 4 subunit B, Firmicutes/Mollicutes / Rossmann fold - #670 / DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat / DNA gyrase/topoisomerase IV, subunit A, C-terminal / DNA gyrase C-terminal domain, beta-propeller / DNA topoisomerase, type IIA, subunit B / DNA topoisomerase, type IIA, alpha-helical domain superfamily / DNA topoisomerase, type IIA, domain A / DNA topoisomerase, type IIA, domain A, alpha-beta / DNA gyrase/topoisomerase IV, subunit A / DNA Topoisomerase IV / DNA gyrase B subunit, C-terminal / DNA gyrase B subunit, carboxyl terminus / DNA topoisomerase, type IIA, subunit B, domain 2 / DNA gyrase B / DNA topoisomerase, type IIA / DNA topoisomerase, type IIA, conserved site / DNA topoisomerase II signature. / TopoisomeraseII / DNA topoisomerase, type IIA, subunit B, C-terminal / Toprim domain / DNA topoisomerase, type IIA-like domain superfamily / Ribosomal Protein S5; domain 2 - #10 / Toprim domain profile. / TOPRIM domain / Ribosomal Protein S5; domain 2 / Histidine kinase-like ATPase, C-terminal domain / Heat Shock Protein 90 / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S5 domain 2-type fold / Rossmann fold / 2-Layer Sandwich / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / DNA topoisomerase 4 subunit A / DNA topoisomerase 4 subunit B / DNA topoisomerase 4 subunit B
Similarity search - Component
Biological speciesStreptococcus pneumoniae (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.7 Å
AuthorsLaponogov, I. / Veselkov, D.A. / Pan, X.-S. / Crevel, I. / Fisher, L.M. / Sanderson, M.R.
CitationJournal: Nucleic Acids Res. / Year: 2013
Title: Structure of an 'open' clamp type II topoisomerase-DNA complex provides a mechanism for DNA capture and transport.
Authors: Laponogov, I. / Veselkov, D.A. / Crevel, I.M. / Pan, X.S. / Fisher, L.M. / Sanderson, M.R.
History
DepositionNov 26, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 28, 2013Provider: repository / Type: Initial release
Revision 1.1Sep 4, 2013Group: Database references
Revision 1.2Dec 4, 2013Group: Database references
Revision 1.3Jul 26, 2017Group: Data collection / Refinement description / Source and taxonomy
Category: diffrn_detector / entity_src_gen / software / Item: _diffrn_detector.detector
Revision 1.4Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: DNA (5'-D(*CP*AP*AP*AP*GP*GP*CP*GP*GP*TP*AP*AP*TP*AP*CP*GP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*GP*AP*AP*TP*CP*AP*GP*G)-3')
F: DNA (5'-D(*CP*CP*TP*GP*AP*TP*TP*CP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*CP*GP*TP*AP*TP*TP*AP*CP*CP*GP*CP*CP*TP*TP*TP*G)-3')
A: Topoisomerase IV subunit B, DNA topoisomerase 4 subunit A chimera
B: Topoisomerase IV subunit B, DNA topoisomerase 4 subunit A chimera
G: DNA (5'-D(*CP*AP*AP*AP*GP*GP*CP*GP*GP*TP*AP*AP*TP*AP*CP*GP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*GP*AP*AP*TP*CP*AP*GP*G)-3')
H: DNA (5'-D(*CP*CP*TP*GP*AP*TP*TP*CP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*CP*GP*TP*AP*TP*TP*AP*CP*CP*GP*CP*CP*TP*TP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)298,5569
Polymers298,4836
Non-polymers733
Water362
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)160.600, 160.600, 280.560
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number94
Space group name H-MP42212
DetailsAsymmetric unit consists of one biological dimer formed by two chains: A and B

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Components

#1: DNA chain DNA (5'-D(*CP*AP*AP*AP*GP*GP*CP*GP*GP*TP*AP*AP*TP*AP*CP*GP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*GP*AP*AP*TP*CP*AP*GP*G)-3')


Mass: 10525.804 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: V-site DNA
#2: DNA chain DNA (5'-D(*CP*CP*TP*GP*AP*TP*TP*CP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*CP*GP*TP*AP*TP*TP*AP*CP*CP*GP*CP*CP*TP*TP*TP*G)-3')


Mass: 10391.674 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: V-site DNA
#3: Protein Topoisomerase IV subunit B, DNA topoisomerase 4 subunit A chimera /


Mass: 128323.859 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Streptococcus pneumoniae (bacteria) / Strain: TIGR4 / Gene: parE, parC / Production host: Escherichia coli (E. coli)
References: UniProt: Q3HZ71, UniProt: D6ZLV0, UniProt: Q59961*PLUS, EC: 5.99.1.-, EC: 5.99.1.3
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.02 Å3/Da / Density % sol: 59.34 %
Crystal growTemperature: 296 K / Method: vapor diffusion, sitting drop / pH: 5
Details: 4% isopropanol, 50 mM sodium citrate, 600 nL + 400 nL (protein + precipitant) drop ratio, VAPOR DIFFUSION, SITTING DROP, temperature 296K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jul 23, 2012
RadiationMonochromator: Single bounce monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.92 Å / Relative weight: 1
ReflectionResolution: 3.7→72.19 Å / Num. all: 39884 / Num. obs: 39884 / % possible obs: 99.75 % / Observed criterion σ(F): -1 / Observed criterion σ(I): -1 / Redundancy: 12.11 % / Rmerge(I) obs: 0.124 / Rsym value: 0.124 / Net I/σ(I): 3.1256
Reflection shellResolution: 3.7→3.9 Å / Redundancy: 11.46 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 1.71 / % possible all: 100

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Processing

Software
NameVersionClassification
GDEdata collection
PHASERphasing
PHENIX(phenix.refine: 1.7.2_869)refinement
MOSFLMdata reduction
SCALAdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3RAE,1EI1

3rae

1ei1


Resolution: 3.7→71.823 Å / SU ML: 0.87 / σ(F): 1.34 / Phase error: 21.92 / Stereochemistry target values: ML
Details: The second DNA dimer (chains E&F) has two alternative orientations related by crystallographic symmetry.
RfactorNum. reflection% reflection
Rfree0.2485 2006 5.04 %
Rwork0.1855 --
obs0.1886 39825 99.69 %
all-39884 -
Solvent computationShrinkage radii: 1.11 Å / VDW probe radii: 1.3 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 66.569 Å2 / ksol: 0.262 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-7.8718 Å20 Å2-0 Å2
2--7.8718 Å2-0 Å2
3----12.19 Å2
Refinement stepCycle: LAST / Resolution: 3.7→71.823 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms14849 1374 3 2 16228
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00916631
X-RAY DIFFRACTIONf_angle_d1.39622931
X-RAY DIFFRACTIONf_dihedral_angle_d18.9035783
X-RAY DIFFRACTIONf_chiral_restr0.0872700
X-RAY DIFFRACTIONf_plane_restr0.0052783
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.7-3.79250.2421490.18182649X-RAY DIFFRACTION100
3.7925-3.89510.24461390.17912672X-RAY DIFFRACTION100
3.8951-4.00970.2381540.1682636X-RAY DIFFRACTION100
4.0097-4.13910.23861290.16352683X-RAY DIFFRACTION100
4.1391-4.2870.23291350.15592655X-RAY DIFFRACTION100
4.287-4.45860.24421530.14682681X-RAY DIFFRACTION100
4.4586-4.66150.19231270.13982670X-RAY DIFFRACTION100
4.6615-4.90720.24781490.14682689X-RAY DIFFRACTION100
4.9072-5.21460.21141380.1642642X-RAY DIFFRACTION98
5.2146-5.61710.2691540.17732688X-RAY DIFFRACTION100
5.6171-6.18210.26281340.19722730X-RAY DIFFRACTION100
6.1821-7.07590.28411470.19572738X-RAY DIFFRACTION100
7.0759-8.91230.24521470.18232792X-RAY DIFFRACTION100
8.9123-71.83610.27291510.2552894X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.9102-0.8765-3.36981.35932.69848.64720.1163-0.59970.22490.1219-0.0393-0.1923-0.58140.301-0.01360.3896-0.0099-0.32580.54820.10880.393972.2768-48.804971.2692
21.21870.58180.75050.44610.27751.6576-0.0587-0.22790.19320.1034-0.32490.0425-0.56380.1070.07010.9625-0.3042-0.20031.1693-0.27110.321159.4517-36.001394.8546
30.4189-0.8910.58725.3937-1.66580.8717-0.3092-0.84750.11510.25550.33420.4664-0.1033-0.5034-0.05330.270.0859-0.02450.70170.33070.649267.6939-38.027639.2041
40.26550.3311-0.3140.4743-0.25110.67940.21390.01610.5123-0.09890.0602-0.3441-0.27430.0441-0.11010.22410.03240.18230.33290.24060.636870.8759-21.539530.0523
52.5508-0.23561.02292.31260.0882.0103-0.0428-0.31940.0409-0.2095-0.33010.6463-0.2484-0.61050.25350.34440.2303-0.16660.5201-0.05010.443855.4384-49.532950.0803
67.1572-1.3092-5.63341.15151.2614.4885-0.1283-1.3294-0.50020.36550.18490.39980.42130.2875-0.11780.7361-0.0063-0.34720.740.19650.592171.33-53.332383.9828
71.18411.17280.35573.22011.05612.4764-0.25080.2645-0.375-0.03050.23310.1792-0.0477-0.13020.01830.47890.2377-0.13880.636-0.16810.714955.5355-73.976422.8384
82.61310.0931.33031.58581.323.078-0.0209-0.0332-0.43540.0902-0.15040.1353-0.176-0.1036-0.20430.22880.0745-0.14350.53040.25650.465572.6902-64.746257.3981
91.7732-0.6639-0.28531.3284-0.33170.83690.16470.3367-0.2769-0.226-0.3509-0.36020.21770.43260.14130.21540.3052-0.24390.5617-0.06820.593274.5495-59.859741.579
103.0076-0.70073.95130.859-1.12876.444-0.0694-0.9055-0.49410.46360.34460.19560.257-0.2037-0.20150.5610.15470.23830.71170.01730.441524.1503-30.48477.2729
110.39770.0729-0.14291.1759-0.3542.2339-0.0154-0.111-0.03320.1722-0.2632-0.0240.187-0.1483-0.13030.9842-0.2707-0.1281.49260.22740.263743.1466-41.858897.2901
123.69040.1427-2.97736.87620.65883.1906-0.0514-0.1992-0.7716-0.14280.0723-1.12550.16561.0142-0.02120.31760.00070.11670.6448-0.18820.795119.7416-44.024445.5645
130.32940.95980.3263.51351.89221.57110.3956-0.0313-0.65250.45990.16480.0440.5515-0.4363-0.37530.5863-0.29360.02010.64880.09121.097914.7987-61.795239.3844
141.8291-0.4001-0.3981.428-0.68881.9765-0.0716-0.14020.1183-0.3758-0.2112-0.57190.39980.45990.24550.42190.24210.1670.48950.22110.474934.5558-31.915852.2403
151.2388-0.20141.60042.07280.34153.0039-0.4495-1.18330.41670.73160.3062-0.1201-0.4211-0.36320.12581.05770.25580.31611.047-0.11190.528327.4219-24.730687.5527
160.63150.36860.08192.13-0.73111.21520.06060.94640.4983-0.3007-0.00140.22380.03970.29980.00920.430.2710.11160.87760.48380.631826.8335-9.45924.246
172.8145-0.0625-0.17492.00830.44352.8325-0.1395-0.75010.61820.1160.00870.07490.2306-0.31290.13350.3280.13930.0460.5738-0.02160.410820.0351-15.867362.1065
182.2303-0.392-0.11911.06510.21141.25190.01830.22930.1131-0.061-0.11220.2581-0.0083-0.21260.13220.24680.19820.20350.43980.13960.534513.7668-21.92548.323
192.9349-3.724-1.46245.17691.51175.1744-0.1186-1.08150.46570.877-0.2178-1.3345-0.10541.32290.35180.59650.04-0.16341.65630.51750.87759.7942-19.222949.1705
205.183-1.6043-0.60772.22150.45851.60460.16960.6660.2235-0.2621-0.3909-0.1704-0.01330.20680.00530.3930.28610.19510.93950.25130.467476.4094-33.574824.9797
211.5831-0.2888-0.1471.5595-0.20230.83790.13040.32960.30690.0951-0.07060.1581-0.1145-0.0689-0.09130.17090.20820.12920.68790.34680.460857.228-21.564725.6753
221.5952-0.82921.92684.37940.22573.4754-0.08-1.4575-0.16210.66580.02571.3629-0.0021-1.08320.02520.41290.07990.05831.5754-0.37570.851128.6383-60.175352.7087
232.2842-0.28640.39390.3477-0.27280.67820.10720.0679-0.2509-0.0739-0.0250.1860.0839-0.08790.0920.28690.1475-0.2440.7457-0.19971.06287.8948-49.014434.668
242.634-0.4395-0.30961.8029-0.11960.81470.44270.0966-0.3608-0.2324-0.20790.25270.13190.0982-0.07450.130.2427-0.32280.4231-0.23620.598726.5927-61.542130.9116
254.89861.06-0.49840.2219-0.11180.02650.31021.7318-0.1392-0.4331-0.22060.3120.115-0.1288-0.09960.57970.3789-0.06071.15650.00180.695342.1001-41.489429.4742
261.2055-1.026-0.01732.2110.05242.76860.04-0.20270.15070.5084-0.3802-0.066-0.1364-0.78270.22690.49610.0054-0.17040.998-0.00590.147421.191527.634323.0567
272.6886-2.36890.65343.0139-0.04081.8935-0.1077-0.7153-0.53980.42470.3042-0.13230.36140.4709-0.17740.53390.26490.00960.85390.16670.590261.9114-111.643522.3869
281.4015-0.7720.27692.44710.21392.36430.13820.260.1495-0.2982-0.2647-0.03730.32280.61390.09920.55680.13860.01250.75610.40880.490941.12765.65594.6774
292.609-0.3977-0.75863.23340.08872.815-0.5705-0.2121-0.12480.31120.2110.0262-0.5991-0.33280.33160.63360.1782-0.22990.60870.01690.603738.7171-89.26478.4458
302.24530.96073.88230.91381.7676.7316-0.15040.09630.8775-0.03080.62240.0053-0.0358-0.0549-0.44051.96290.14280.1841.6066-0.0271.393679.1371-79.825266.0498
312.40721.2409-3.94070.6392-2.02486.4598-0.2205-0.0292-0.95110.03970.4705-0.0675-0.0076-0.1274-0.2961.80020.1587-0.14251.49820.07061.476381.6578-80.895866.0264
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain "A" and resseq 1343:1382A1343 - 1382
2X-RAY DIFFRACTION2chain "A" and resseq 1383:1429A1383 - 1429
3X-RAY DIFFRACTION3chain "A" and resseq 1018:1030A1018 - 1030
4X-RAY DIFFRACTION4chain "A" and resseq 1003:1017A1003 - 1017
5X-RAY DIFFRACTION5chain "A" and resseq 1031:1154A1031 - 1154
6X-RAY DIFFRACTION6chain "A" and resseq 1430:1455A1430 - 1455
7X-RAY DIFFRACTION7chain "A" and resseq 1239:1322A1239 - 1322
8X-RAY DIFFRACTION8chain "A" and resseq 1456:1482A1456 - 1482
9X-RAY DIFFRACTION9chain "A" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 or resid 1901 )A1155 - 1238
10X-RAY DIFFRACTION9chain "A" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 or resid 1901 )A1323 - 1342
11X-RAY DIFFRACTION9chain "A" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 or resid 1901 )A1483 - 1484
12X-RAY DIFFRACTION10chain "B" and resseq 1343:1382B1343 - 1382
13X-RAY DIFFRACTION11chain "B" and resseq 1383:1429B1383 - 1429
14X-RAY DIFFRACTION12chain "B" and resseq 1018:1030B1018 - 1030
15X-RAY DIFFRACTION13chain "B" and resseq 1002:1017B1002 - 1017
16X-RAY DIFFRACTION14chain "B" and resseq 1031:1154B1031 - 1154
17X-RAY DIFFRACTION15chain "B" and resseq 1430:1455B1430 - 1455
18X-RAY DIFFRACTION16chain "B" and resseq 1239:1322B1239 - 1322
19X-RAY DIFFRACTION17chain "B" and resseq 1456:1482B1456 - 1482
20X-RAY DIFFRACTION18chain "B" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 )B1155 - 1238
21X-RAY DIFFRACTION18chain "B" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 )B1323 - 1342
22X-RAY DIFFRACTION18chain "B" and ( resseq 1155:1238 or resseq 1323:1342 or resseq 1483:1484 or resid 1700 )B1483 - 1484
23X-RAY DIFFRACTION19chain "A" and resseq 539:581A539 - 581
24X-RAY DIFFRACTION20chain "A" and resseq 610:634A610 - 634
25X-RAY DIFFRACTION21chain "A" and ( resseq 414:538 or resseq 582:609 or resseq 635:642 )A414 - 538
26X-RAY DIFFRACTION21chain "A" and ( resseq 414:538 or resseq 582:609 or resseq 635:642 )A582 - 609
27X-RAY DIFFRACTION21chain "A" and ( resseq 414:538 or resseq 582:609 or resseq 635:642 )A635 - 642
28X-RAY DIFFRACTION22chain "B" and resseq 539:581B539 - 581
29X-RAY DIFFRACTION23chain "B" and resseq 610:634B610 - 634
30X-RAY DIFFRACTION24chain "B" and ( resseq 414:538 or resseq 582:609 or resseq 635:640 )B414 - 538
31X-RAY DIFFRACTION24chain "B" and ( resseq 414:538 or resseq 582:609 or resseq 635:640 )B582 - 609
32X-RAY DIFFRACTION24chain "B" and ( resseq 414:538 or resseq 582:609 or resseq 635:640 )B635 - 640
33X-RAY DIFFRACTION25chain "G" or chain "H"G8 - 27
34X-RAY DIFFRACTION26chain A and resid 24:220A24 - 220
35X-RAY DIFFRACTION27chain B and resid 20:220B20 - 220
36X-RAY DIFFRACTION28chain A and resid 221:396A221 - 396
37X-RAY DIFFRACTION29chain B and resid 221:396B221 - 396
38X-RAY DIFFRACTION30chain EE2 - 15
39X-RAY DIFFRACTION31chain FF2 - 15

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