PDB-4o7m: Crystal structure of a trap periplasmic solute binding protein fr...

ID or keywords:

Entry

Database: PDB / ID: 4o7m

Title

Crystal structure of a trap periplasmic solute binding protein from shewanella loihica PV-4, target EFI-510273, with bound L-malate

Components

TRAP dicarboxylate transporter, DctP subunit

Keywords

TRANSPORT PROTEIN / TRAP PERIPLASMIC SOLUTE BINDING FAMILY /

ENZYME FUNCTION INITIATIVE / EFI /

Structural Genomics

Function / homology

Function and homology information

organic substance transport / transmembrane transport / outer membrane-bounded periplasmic space
Similarity search - Function

Biological species

Shewanella loihica (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 1.5 Å

Authors

Vetting, M.W. / Al Obaidi, N.F. / Morisco, L.L. / Wasserman, S.R. / Sojitra, S. / Stead, M. / Attonito, J.D. / Scott Glenn, A. / Chowdhury, S. / Evans, B. ...

Citation

Journal: Biochemistry / Year: 2015
Title: Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Authors: Vetting, M.W. / Al-Obaidi, N. / Zhao, S. / San Francisco, B. / Kim, J. / Wichelecki, D.J. / Bouvier, J.T. / Solbiati, J.O. / Vu, H. / Zhang, X. / Rodionov, D.A. / Love, J.D. / Hillerich, B.S. ...

History

Deposition	Dec 25, 2013	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Mar 5, 2014	Provider: repository / Type: Initial release
Revision 1.1	Feb 25, 2015	Group: Database references

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4o7m.cif.gz	715.6 KB	Display	PDBx/mmCIF format
PDB format	pdb4o7m.ent.gz	629.9 KB	Display	PDB format
PDBx/mmJSON format	4o7m.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/o7/4o7m ftp://data.pdbj.org/pub/pdb/validation_reports/o7/4o7m	HTTPS FTP

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Related structure data

Related structure data	4ln5C 4mcoC 4mevC 4mhfC 4mijC 4mncC 4mniC 4mx6C 4n15C 4n17C 4n4uC 4n6dC 4n6kC 4n8gC 4n8yC 4n91C 4napC 4nf0C 4ng7C 4nguC 4nhbC 4nn3C 4nq8C 4nx1C 4o8mC 4o94C 4oa4C 4oanC 4ovpC 4ovqC 4ovrC 4ovsC 4ovtC 4p1eC 4p1lC 4p3lC 4p47C 4p56C 4p8bC 4p9kC 4pafC 4paiC 4pakC 4pbhC 4pbqC 4pc9C 4pcdC 4pddC 4pdhC 4pe3C 4petC 4pf6C 4pf8C 4pfbC 4pfiC 4pfrC 4pgnC 4pgpC 4uabC C: citing same article (ref.)
Similar structure data	4oa4-2 4oa4-4 6ntp-d 2vpn-1 4nhb-1 4ovp-2 5hu3-d 4xeq-3 3bkz-d 4xeq-4 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Other databases	TargetTrack: EFI-510273

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#190 - Oct 2015 Two-component Systems similarity (1)

Deposited unit

A: TRAP dicarboxylate transporter, DctP subunit

B: TRAP dicarboxylate transporter, DctP subunit

C: TRAP dicarboxylate transporter, DctP subunit

D: TRAP dicarboxylate transporter, DctP subunit

hetero molecules

150 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: TRAP dicarboxylate transporter, DctP subunit

hetero molecules

defined by author&software
37.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: TRAP dicarboxylate transporter, DctP subunit

hetero molecules

defined by author&software
37.6 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: TRAP dicarboxylate transporter, DctP subunit

hetero molecules

defined by author&software
37.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: TRAP dicarboxylate transporter, DctP subunit

hetero molecules

defined by author&software
37.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	80.515, 118.640, 89.901
Angle α, β, γ (deg.)	90.00, 93.18, 90.00
Int Tables number	4
Space group name H-M	P12₁1

#1: Protein	TRAP dicarboxylate transporter, DctP subunit Mass: 37388.996 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Shewanella loihica (bacteria) / Strain: PV-4 / Gene: Shew_1446 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A3QCW5
#2: Chemical	ChemComp-LMR / (2S)-2-hydroxybutanedioic acid / L-Malate / Malic acid Mass: 134.087 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₄H₆O₅
#3: Chemical	ChemComp-SO4 / SULFATE ION / Sulfate Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO₄
#4: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 2229 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.87 Å³/Da / Density % sol: 57.09 %
Crystal grow	Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein (34.4 mg/ml, 10 mM HEPES, 5 mM DTT, 10 mM L-Malate); Reservoir (0.2 M Sodium Citrate, 20% (w/v) PEG 3350); Cryoprotection (Reservoir with 20% Diethylene Glycol), pH 7.5, VAPOR ...Details: Protein (34.4 mg/ml, 10 mM HEPES, 5 mM DTT, 10 mM L-Malate); Reservoir (0.2 M Sodium Citrate, 20% (w/v) PEG 3350); Cryoprotection (Reservoir with 20% Diethylene Glycol), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystal

Density Matthews: 2.87 Å³/Da / Density % sol: 57.09 %

Crystal grow

Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: Protein (34.4 mg/ml, 10 mM HEPES, 5 mM DTT, 10 mM L-Malate); Reservoir (0.2 M Sodium Citrate, 20% (w/v) PEG 3350); Cryoprotection (Reservoir with 20% Diethylene Glycol), pH 7.5, VAPOR ...

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 31-ID / Wavelength: 0.9793 Å

Detector

Type: RAYONIX 225 HE / Detector: CCD / Date: Nov 8, 2013 / Details: MIRRORS

Radiation

Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9793 Å / Relative weight: 1

Reflection

Resolution: 1.5→118.64 Å / Num. all: 268529 / Num. obs: 268529 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.6 % / B_iso Wilson estimate: 9.42 Å² / R_merge(I) obs: 0.121 / R_sym value: 0.121 / Net I/σ(I): 10.5

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. measured all	Num. unique all	R_sym value	% possible all
1.5-1.58	7.4	0.601	1.3	288028	39173	0.601	100
1.58-1.68	7.5	0.447	1.7	276274	37014	0.447	100
1.68-1.79	7.6	0.319	2.4	263078	34837	0.319	100
1.79-1.94	7.6	0.223	3.4	246551	32406	0.223	100
1.94-2.12	7.6	0.161	3.6	228218	29853	0.161	100
2.12-2.37	7.7	0.127	5.3	207592	27011	0.127	100
2.37-2.74	7.7	0.102	5.6	183743	23808	0.102	100
2.74-3.35	7.7	0.072	8.2	156467	20208	0.072	100
3.35-4.74	7.8	0.056	10.1	121371	15652	0.056	100
4.74-22.603	7.6	0.055	9.9	65502	8567	0.055	99

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Processing

Software

Name	Version	Classification
MOSFLM		data reduction
SCALA	3.3.20	data scaling
PHENIX	1.8.1_1168	refinement
PDB_EXTRACT	3.14	data extraction
PHENIX	AUTOSOLVE	phasing

Refinement

Method to determine structure:

SAD / Resolution: 1.5→22.603 Å / Occupancy max: 1 / Occupancy min: 0.21 / FOM work R set: 0.93 / SU ML: 0.11 / σ(F): 0 / σ(I): 0 / Phase error: 13.87 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.1575	13518	5.03 %	RANDOM
R_work	0.1381	-	-	-
all	0.1391	268483	-	-
obs	0.1391	268483	100 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 53.69 Å² / B_iso mean: 13.7201 Å² / B_iso min: 2.87 Å²

Refinement step

Cycle: LAST / Resolution: 1.5→22.603 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	9455	0	41	2229	11725

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.01	10016
X-RAY DIFFRACTION	f_angle_d	1.221	13567
X-RAY DIFFRACTION	f_chiral_restr	0.084	1491
X-RAY DIFFRACTION	f_plane_restr	0.007	1779
X-RAY DIFFRACTION	f_dihedral_angle_d	13.803	3763

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30 / % reflection obs: 100 %

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all
1.5-1.517	0.2336	447	0.2114	8520	8967
1.517-1.5349	0.2116	422	0.1995	8437	8859
1.5349-1.5536	0.2111	446	0.1908	8490	8936
1.5536-1.5733	0.2072	444	0.1804	8499	8943
1.5733-1.594	0.2022	458	0.1762	8440	8898
1.594-1.6158	0.1916	446	0.1659	8486	8932
1.6158-1.6389	0.1805	448	0.1626	8484	8932
1.6389-1.6633	0.1775	433	0.1573	8518	8951
1.6633-1.6893	0.1807	448	0.1512	8463	8911
1.6893-1.717	0.1629	458	0.1433	8473	8931
1.717-1.7466	0.1606	446	0.1379	8519	8965
1.7466-1.7783	0.1661	480	0.1357	8410	8890
1.7783-1.8125	0.1659	434	0.1367	8504	8938
1.8125-1.8495	0.1693	434	0.132	8486	8920
1.8495-1.8897	0.1477	509	0.126	8452	8961
1.8897-1.9336	0.154	454	0.1273	8476	8930
1.9336-1.982	0.1594	473	0.1273	8478	8951
1.982-2.0355	0.1503	443	0.1258	8481	8924
2.0355-2.0954	0.1487	439	0.1262	8524	8963
2.0954-2.163	0.1502	457	0.1244	8481	8938
2.163-2.2402	0.1425	453	0.121	8518	8971
2.2402-2.3298	0.1348	441	0.1189	8452	8893
2.3298-2.4357	0.1407	457	0.1201	8541	8998
2.4357-2.564	0.1422	455	0.1219	8507	8962
2.564-2.7244	0.141	441	0.1254	8505	8946
2.7244-2.9343	0.1582	446	0.1367	8504	8950
2.9343-3.2288	0.1587	460	0.1382	8548	9008
3.2288-3.6943	0.1454	434	0.1347	8549	8983
3.6943-4.6478	0.1302	489	0.1225	8542	9031
4.6478-22.6058	0.1748	423	0.1619	8678	9101

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.7657	-0.2525	-0.0824	0.4215	0.0994	0.2429	-0.0201	0.0148	0.1705	-0.0716	0.0341	0.0603	-0.0658	-0.016	-0.0199	0.0747	-0.0056	-0.0117	0.0372	0.0049	0.0908	70.911	72.092	66.008
2	0.3091	0.0519	-0.1736	0.9509	-0.1884	0.2062	-0.0099	-0.0561	-0.0155	0.0471	-0.0099	-0.0049	0.0233	0.0243	0.0186	0.046	-0.0115	-0.0059	0.0481	0.0012	0.0482	73.96	56.485	76.297
3	0.5156	0.051	0.0352	0.6417	-0.0743	0.6436	-0.02	0.0119	-0.0322	-0.0847	-0.0232	-0.0607	0.1192	0.1191	0.0344	0.0756	0.0117	0.0183	0.064	0.008	0.0639	85.54	49.743	59.288
4	0.3337	-0.358	-0.2027	1.8333	0.8178	0.7276	-0.0157	-0.0884	0.0536	0.1784	-0.0344	-0.0308	0.0884	-0.0345	0.007	0.0478	-0.0087	-0.0032	0.062	-0.0184	0.0725	71.426	66.028	77.055
5	0.846	-0.6165	-0.8783	1.3228	1.3445	1.9856	0.0028	0.0349	0.0512	-0.037	0.0073	0.0498	0.0084	-0.0483	0.0275	0.0506	-0.0076	-0.0208	0.0468	0.0003	0.0761	65.018	61.014	63.154
6	0.277	-0.033	-0.1357	0.0643	-0.0691	0.7059	-0.0386	-0.0342	-0.2252	-0.1497	-0.0083	0.0676	0.2963	0.0636	0.0186	0.1361	-0.0012	0.0072	0.0573	0.0298	0.1012	78.472	39.829	67.994
7	0.5338	0.0443	0.0093	0.405	0.1083	0.5436	0.0054	0.0017	-0.1196	-0.0035	-0.007	0.0448	0.0745	-0.0642	-0.0055	0.0545	0.008	0.0043	0.05	-0.0026	0.077	34.936	34.661	65.033
8	0.3244	0.3034	-0.0524	1.4541	0.4242	1.6881	0.0195	0.0604	0.072	-0.08	-0.0418	-0.0975	-0.0728	0.0103	0.0274	0.0513	0.0203	-0.0015	0.0566	0.013	0.0602	38.4	56.166	58.666
9	0.3992	0.2117	0.3012	0.4237	0.2935	0.5132	-0.0063	0.0128	0.086	-0.0765	-0.0098	0.0057	-0.069	-0.0477	-0.0017	0.0731	0.0276	-0.0011	0.0791	0.0056	0.0864	33.945	55.246	62.208
10	0.3171	0.0428	0.0594	0.3442	0.0261	0.1742	-0.0106	0.0136	0.024	-0.0224	-0.0139	-0.0226	-0.0165	-0.0217	0.0259	0.0407	0.0148	0.0009	0.0487	0.0028	0.0642	43.132	52.457	70.27
11	0.6482	0.4481	0.6381	1.3056	1.2996	1.9987	0.0229	-0.0286	-0.0342	0.0264	-0.0227	0.0791	0.0453	-0.1593	0.006	0.0387	0.0037	0.0067	0.0913	-0.0013	0.0823	25.982	44.225	69.783
12	0.3241	0.0437	0.1617	0.2599	0.0785	0.8975	-0.0369	0.0103	0.1711	-0.021	0.0032	0.0059	-0.1545	-0.0066	0.0072	0.095	0.0367	0.0003	0.0917	0.0222	0.1594	37.021	66.878	65.733
13	0.4531	0.1356	0.0308	0.4504	0.041	0.8354	0.029	-0.1518	0.0604	0.21	-0.0447	0.0494	-0.106	-0.0087	0.0237	0.0851	0.0027	0.0109	0.0778	0.0036	0.0684	50.744	40.52	96.239
14	0.57	0.0464	-0.0363	0.3239	-0.2339	0.278	0.0162	-0.0207	0.0328	0.0162	-0.0187	-0.0014	-0.0992	-0.0096	0.0038	0.0468	0.0001	0.0015	0.0405	0.013	0.047	56.042	41.794	88.104
15	1.0883	0.1853	0.1878	1.2993	0.2748	0.3409	0.0251	0.0935	0.0053	-0.0965	-0.0212	-0.1058	0.066	0.0037	0.0143	0.0469	0.0148	0.0044	0.0702	0.0324	0.0561	61.588	20.706	86.206
16	0.4069	0.0626	0.1481	0.4854	0.0343	0.5655	0.0144	0.0101	-0.0259	-0.0057	-0.0281	-0.0769	0.0704	-0.0028	-0.0012	0.0614	0.009	0.0028	0.0654	0.033	0.075	57.834	20.762	90.757
17	1.6573	0.0587	0.2305	2.0064	0.3407	1.1881	-0.0087	0.0573	0.0115	-0.0809	-0.0489	-0.0557	0.0526	0.0027	0.0443	0.0914	0.0011	0.009	0.0717	0.0268	0.047	52.85	16.724	75.827
18	0.7983	-0.4753	-0.0899	0.9308	0.025	0.8079	0.0521	0.1303	0.0198	-0.1613	-0.0146	0.0846	0.0105	-0.1088	-0.0134	0.0616	-0.0079	-0.0127	0.0773	0.0304	0.053	45.092	22.996	75.357
19	0.4233	0.2337	0.0942	0.5251	0.0092	0.4652	0.0062	-0.042	0.0191	-0.0002	-0.0239	0.0395	0.0063	-0.0632	0.0059	0.0278	0.0013	-0.0007	0.0531	0.0224	0.0473	50.416	27.192	88.867
20	1.5436	1.0936	0.5775	1.4749	0.4197	0.5433	-0.0177	-0.1205	-0.0443	0.1388	-0.014	0.0275	-0.0539	-0.1177	0.0075	0.0821	-0.0257	0.0226	0.1113	0.0046	0.041	49.57	30.183	99.865
21	0.7768	0.0811	0.2208	0.211	0.0798	0.3543	0.0119	0.0407	-0.1084	0.0019	-0.0048	0.01	0.2388	-0.0237	-0.0197	0.1004	0.0043	0.0082	0.082	0.0399	0.0928	55.577	9.659	86.427
22	0.3909	0.0676	-0.0447	0.3854	-0.193	0.9114	-0.1126	-0.1221	-0.0979	0.2198	0.0841	-0.0836	0.1647	0.0674	0.0266	0.1494	0.0455	0.0045	0.1028	-0.0017	0.0701	65.195	8.28	48.745
23	0.3153	-0.1174	-0.0634	1.9225	-0.0267	0.1679	-0.009	0.0262	-0.0497	0.0075	-0.0237	0.1527	0.0289	-0.0191	0.0314	0.0606	0.0011	0.0109	0.0687	-0.0256	0.0561	53.944	20.064	40.496
24	0.6886	0.1153	-0.2141	1.7991	0.3577	0.7964	-0.0878	0.0239	-0.0232	0.1222	0.0273	0.1878	0.0092	-0.0182	0.0498	0.1036	0.0187	0.0254	0.0806	-0.0223	0.0816	50.778	23.107	49.592
25	0.5371	-0.1138	0.1494	0.6032	0.0529	0.5594	-0.0353	0.0408	0.0155	-0.0378	0.0226	-0.0608	-0.0277	0.0665	0.0131	0.08	-0.0059	0.0039	0.0954	-0.015	0.0537	67.575	29.734	31.905
26	0.3971	0.2091	-0.1274	0.9086	-0.2026	0.3863	-0.0698	-0.0335	-0.0036	0.0428	0.065	0.13	0.0664	-0.0043	0.0155	0.0874	0.009	0.0115	0.0774	-0.0198	0.0542	58.61	19.717	44.544
27	0.9576	0.7773	-0.5822	1.341	-0.6959	0.7313	-0.0225	-0.1685	0.0003	0.2994	0.0418	-0.0797	0.0244	0.0556	-0.0098	0.1673	0.0378	0.002	0.1293	-0.0141	0.0344	66.253	20.184	53.833
28	0.6197	0.1425	-0.1879	0.3101	0.019	0.4089	-0.0487	0.0967	0.1025	-0.0127	0.0226	0.0493	-0.1532	-0.0391	-0.0014	0.0939	0.009	-0.0177	0.0778	-0.0316	0.0628	57.786	39.113	38.928

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 33:82 )	A	33 - 82
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 83:152 )	A	83 - 152
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 153:238 )	A	153 - 238
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 239:256 )	A	239 - 256
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 257:292 )	A	257 - 292
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 293:336 )	A	293 - 336
7	X-RAY DIFFRACTION	7	( CHAIN B AND RESID 32:98 )	B	32 - 98
8	X-RAY DIFFRACTION	8	( CHAIN B AND RESID 99:120 )	B	99 - 120
9	X-RAY DIFFRACTION	9	( CHAIN B AND RESID 121:170 )	B	121 - 170
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 171:256 )	B	171 - 256
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 257:292 )	B	257 - 292
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 293:336 )	B	293 - 336
13	X-RAY DIFFRACTION	13	( CHAIN C AND RESID 32:62 )	C	32 - 62
14	X-RAY DIFFRACTION	14	( CHAIN C AND RESID 63:98 )	C	63 - 98
15	X-RAY DIFFRACTION	15	( CHAIN C AND RESID 99:120 )	C	99 - 120
16	X-RAY DIFFRACTION	16	( CHAIN C AND RESID 121:170 )	C	121 - 170
17	X-RAY DIFFRACTION	17	( CHAIN C AND RESID 171:190 )	C	171 - 190
18	X-RAY DIFFRACTION	18	( CHAIN C AND RESID 191:217 )	C	191 - 217
19	X-RAY DIFFRACTION	19	( CHAIN C AND RESID 218:256 )	C	218 - 256
20	X-RAY DIFFRACTION	20	( CHAIN C AND RESID 257:292 )	C	257 - 292
21	X-RAY DIFFRACTION	21	( CHAIN C AND RESID 293:312 )	C	293 - 312
22	X-RAY DIFFRACTION	22	( CHAIN D AND RESID 33:82 )	D	33 - 82
23	X-RAY DIFFRACTION	23	( CHAIN D AND RESID 83:120 )	D	83 - 120
24	X-RAY DIFFRACTION	24	( CHAIN D AND RESID 121:152 )	D	121 - 152
25	X-RAY DIFFRACTION	25	( CHAIN D AND RESID 153:229 )	D	153 - 229
26	X-RAY DIFFRACTION	26	( CHAIN D AND RESID 230:256 )	D	230 - 256
27	X-RAY DIFFRACTION	27	( CHAIN D AND RESID 257:292 )	D	257 - 292
28	X-RAY DIFFRACTION	28	( CHAIN D AND RESID 293:335 )	D	293 - 335

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