7QQZ
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![BU of 7qqz by Molmil](/molmil-images/mine/7qqz) | SpCas9 bound to FANCF off-target7 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target7 non-target strand, FANCF off-target7 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.25 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQW
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![BU of 7qqw by Molmil](/molmil-images/mine/7qqw) | SpCas9 bound to FANCF off-target4 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target4 non-target strand, FANCF off-target4 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR1
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![BU of 7qr1 by Molmil](/molmil-images/mine/7qr1) | SpCas9 bound to TRAC off-target2 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR7
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![BU of 7qr7 by Molmil](/molmil-images/mine/7qr7) | SpCas9 bound to AAVS1 off-target2 DNA substrate | Descriptor: | AAVS1 off-target2 non-target strand, AAVS1 off-target2 target strand, AAVS1 sgRNA, ... | Authors: | Pacesa, M, JInek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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5EEH
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![BU of 5eeh by Molmil](/molmil-images/mine/5eeh) | Crystal structure of carminomycin-4-O-methyltransferase DnrK in complex with SAH and 2-chloro-4-nitrophenol | Descriptor: | 2-chloranyl-4-nitro-phenol, Carminomycin 4-O-methyltransferase DnrK, S-ADENOSYL-L-HOMOCYSTEINE, ... | Authors: | Wang, F, Singh, S, Thorson, J.S, Phillips Jr, G.N, Enzyme Discovery for Natural Product Biosynthesis (NatPro) | Deposit date: | 2015-10-22 | Release date: | 2015-12-16 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.82 Å) | Cite: | Functional AdoMet Isosteres Resistant to Classical AdoMet Degradation Pathways. Acs Chem.Biol., 11, 2016
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7S4V
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![BU of 7s4v by Molmil](/molmil-images/mine/7s4v) | Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, NTS, TS, ... | Authors: | Bravo, J.P.K, Taylor, D.W, Liu, M.S, Johnson, K.A. | Deposit date: | 2021-09-09 | Release date: | 2022-03-02 | Last modified: | 2024-06-05 | Method: | ELECTRON MICROSCOPY (3.28 Å) | Cite: | Structural basis for mismatch surveillance by CRISPR-Cas9. Nature, 603, 2022
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7S4U
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![BU of 7s4u by Molmil](/molmil-images/mine/7s4u) | Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand, Target strand, ... | Authors: | Bravo, J.P.K, Taylor, D.W, Liu, M.S, Johnson, K.A. | Deposit date: | 2021-09-09 | Release date: | 2022-03-02 | Last modified: | 2024-06-05 | Method: | ELECTRON MICROSCOPY (3.56 Å) | Cite: | Structural basis for mismatch surveillance by CRISPR-Cas9. Nature, 603, 2022
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7S4X
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![BU of 7s4x by Molmil](/molmil-images/mine/7s4x) | Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, NTS, ... | Authors: | Bravo, J.P.K, Taylor, D.W, Liu, M.S, Johnson, K.A. | Deposit date: | 2021-09-09 | Release date: | 2022-03-02 | Last modified: | 2024-06-05 | Method: | ELECTRON MICROSCOPY (2.76 Å) | Cite: | Structural basis for mismatch surveillance by CRISPR-Cas9. Nature, 603, 2022
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5F9R
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![BU of 5f9r by Molmil](/molmil-images/mine/5f9r) | |
7S37
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![BU of 7s37 by Molmil](/molmil-images/mine/7s37) | Cas9:sgRNA (S. pyogenes) in the open-protein conformation | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Single-guide RNA | Authors: | Cofsky, J.C, Soczek, K.M, Knott, G.J, Nogales, E, Doudna, J.A. | Deposit date: | 2021-09-04 | Release date: | 2022-04-20 | Last modified: | 2024-06-05 | Method: | ELECTRON MICROSCOPY (3.2 Å) | Cite: | CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29, 2022
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7S3H
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![BU of 7s3h by Molmil](/molmil-images/mine/7s3h) | Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target DNA strand, Single-guide RNA, ... | Authors: | Cofsky, J.C, Soczek, K.M, Knott, G.J, Nogales, E, Doudna, J.A. | Deposit date: | 2021-09-06 | Release date: | 2022-04-20 | Last modified: | 2022-05-04 | Method: | ELECTRON MICROSCOPY (2.5 Å) | Cite: | CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29, 2022
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7S36
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![BU of 7s36 by Molmil](/molmil-images/mine/7s36) | Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, closed-protein/bent-DNA conformation | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target DNA strand, Single-guide RNA, ... | Authors: | Cofsky, J.C, Soczek, K.M, Knott, G.J, Nogales, E, Doudna, J.A. | Deposit date: | 2021-09-04 | Release date: | 2022-04-20 | Last modified: | 2022-05-04 | Method: | ELECTRON MICROSCOPY (3.2 Å) | Cite: | CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29, 2022
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7S38
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![BU of 7s38 by Molmil](/molmil-images/mine/7s38) | Cas9:sgRNA:DNA (S. pyogenes) forming a 3-base-pair R-loop | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target DNA strand, Single-guide RNA, ... | Authors: | Cofsky, J.C, Soczek, K.M, Knott, G.J, Nogales, E, Doudna, J.A. | Deposit date: | 2021-09-04 | Release date: | 2022-04-20 | Last modified: | 2022-05-04 | Method: | ELECTRON MICROSCOPY (3.3 Å) | Cite: | CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29, 2022
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8PM4
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![BU of 8pm4 by Molmil](/molmil-images/mine/8pm4) | Cryo-EM structure of the Cas12m-crRNA-target DNA complex | Descriptor: | DNA oligoduplex, non-target strand, chain D, ... | Authors: | Sasnauskas, G, Tamulaitiene, G, Karvelis, T, Bigelyte, G, Siksnys, V. | Deposit date: | 2023-06-28 | Release date: | 2024-02-07 | Last modified: | 2024-04-24 | Method: | ELECTRON MICROSCOPY (2.93 Å) | Cite: | Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing. Nucleic Acids Res., 52, 2024
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8ETQ
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![BU of 8etq by Molmil](/molmil-images/mine/8etq) | |
7V59
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![BU of 7v59 by Molmil](/molmil-images/mine/7v59) | Cryo-EM structure of spyCas9-sgRNA-DNA dimer | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (49-MER), RNA (115-MER) | Authors: | Liu, J, Deng, P. | Deposit date: | 2021-08-16 | Release date: | 2022-08-17 | Last modified: | 2024-06-12 | Method: | ELECTRON MICROSCOPY (5.26 Å) | Cite: | Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci, 12, 2021
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5FW3
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![BU of 5fw3 by Molmil](/molmil-images/mine/5fw3) | Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Anders, C, Bargsten, K, Jinek, M. | Deposit date: | 2016-02-11 | Release date: | 2016-06-15 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.7 Å) | Cite: | Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol.Cell, 61, 2016
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8G1I
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![BU of 8g1i by Molmil](/molmil-images/mine/8g1i) | SpCas9 with sgRNA and target DNA | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA E2_NTS, DNA E2_TS, ... | Authors: | Korolev, S, Gagnon, K. | Deposit date: | 2023-02-02 | Release date: | 2023-05-17 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.12 Å) | Cite: | SpCas9 with sgRNA and target DNA To Be Published
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8FZT
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![BU of 8fzt by Molmil](/molmil-images/mine/8fzt) | SpCas9 with dual-guide RNA and target DNA | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, CrE2, E2-NTS, ... | Authors: | Korolev, S, Gagnon, K. | Deposit date: | 2023-01-30 | Release date: | 2023-05-31 | Last modified: | 2024-06-19 | Method: | ELECTRON MICROSCOPY (3.03 Å) | Cite: | SpCas9 with dual-guide RNA and target DNA To Be Published
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2OCZ
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![BU of 2ocz by Molmil](/molmil-images/mine/2ocz) | The Structure of a Putative 3-Dehydroquinate Dehydratase from Streptococcus pyogenes. | Descriptor: | 1,2-ETHANEDIOL, 3-dehydroquinate dehydratase, MAGNESIUM ION | Authors: | Cuff, M.E, Duggan, E, Clancy, S, Joachimiak, A, Midwest Center for Structural Genomics (MCSG) | Deposit date: | 2006-12-21 | Release date: | 2007-01-23 | Last modified: | 2023-12-27 | Method: | X-RAY DIFFRACTION (1.85 Å) | Cite: | The Structure of a Putative 3-Dehydroquinate Dehydratase from Streptococcus pyogenes. TO BE PUBLISHED
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2OHG
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![BU of 2ohg by Molmil](/molmil-images/mine/2ohg) | |
4QL5
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![BU of 4ql5 by Molmil](/molmil-images/mine/4ql5) | Crystal structure of translation initiation factor IF-1 from Streptococcus pneumoniae TIGR4 | Descriptor: | ACETATE ION, GLYCEROL, Translation initiation factor IF-1, ... | Authors: | Stogios, P.J, Wawrzak, Z, Onopriyenko, O, Savchenko, A, Anderson, W.F, Center for Structural Genomics of Infectious Diseases (CSGID) | Deposit date: | 2014-06-10 | Release date: | 2014-07-02 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (2.025 Å) | Cite: | Crystal structure of translation initiation factor IF-1 from Streptococcus pneumoniae TIGR4 TO BE PUBLISHED
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4OO8
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![BU of 4oo8 by Molmil](/molmil-images/mine/4oo8) | Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3'), RNA (97-MER) | Authors: | Nishimasu, H, Ishitani, R, Nureki, O. | Deposit date: | 2014-01-31 | Release date: | 2014-02-26 | Last modified: | 2024-04-03 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Crystal structure of Cas9 in complex with guide RNA and target DNA Cell(Cambridge,Mass.), 156, 2014
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4Q2W
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![BU of 4q2w by Molmil](/molmil-images/mine/4q2w) | Crystal Structure of pneumococcal peptidoglycan hydrolase LytB | Descriptor: | GLYCEROL, Putative endo-beta-N-acetylglucosaminidase | Authors: | Bai, X.H, Chen, H.J, Jiang, Y.L, Wen, Z, Cheng, W, Li, Q, Zhang, J.R, Chen, Y, Zhou, C.Z. | Deposit date: | 2014-04-10 | Release date: | 2014-07-16 | Last modified: | 2019-12-18 | Method: | X-RAY DIFFRACTION (1.65 Å) | Cite: | Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis. J.Biol.Chem., 289, 2014
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6K3Z
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![BU of 6k3z by Molmil](/molmil-images/mine/6k3z) | Crystal structure of dCas9 in complex with sgRNA and DNA (TGA PAM) | Descriptor: | CRISPR-associated endonuclease Cas9, DNA (28-MER), DNA (5'-D(*AP*AP*AP*TP*GP*AP*TP*AP*TP*TP*G)-3'), ... | Authors: | Chen, W, Zhang, H, Zhang, Y, Wang, Y, Gan, J, Ji, Q. | Deposit date: | 2019-05-22 | Release date: | 2019-09-25 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (3.2 Å) | Cite: | Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. Plos Biol., 17, 2019
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