7S4U

Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point

Summary for 7S4U

Entry DOI	10.2210/pdb7s4u/pdb
EMDB information	24833 24834 24835 24836 24837 24838
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, gRNA, Target strand, ... (4 entities in total)
Functional Keywords	cas9, mismatch, immune system-dna-rna complex, immune system/dna/rna
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	206303.02
Authors	Bravo, J.P.K.,Taylor, D.W.,Liu, M.S.,Johnson, K.A. (deposition date: 2021-09-09, release date: 2022-03-02, Last modification date: 2022-03-23)
Primary citation	Bravo, J.P.K.,Liu, M.S.,Hibshman, G.N.,Dangerfield, T.L.,Jung, K.,McCool, R.S.,Johnson, K.A.,Taylor, D.W. Structural basis for mismatch surveillance by CRISPR-Cas9. Nature, 603:343-347, 2022 Cited by PubMed: 35236982 DOI: 10.1038/s41586-022-04470-1 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.56 Å)

Structure validation