7S4U
Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158699.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | gRNA | polymer | 97 | 31357.7 | 1 | synthetic construct | ||
| 3 | C (C) | Target strand | polymer | 36 | 10985.0 | 1 | synthetic construct | ||
| 4 | D (D) | Non-target strand | polymer | 17 | 5260.4 | 1 | synthetic construct |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 206303.0 | |
| All* | Total formula weight | 206303.0 |
