7V59

Cryo-EM structure of spyCas9-sgRNA-DNA dimer

Summary for 7V59

• Entry DOI: 10.2210/pdb7v59/pdb
• EMDB information: 31721
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, RNA (115-MER), DNA (49-MER) (3 entities in total)
• Functional Keywords: complex, rna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 6
• Total formula weight: 420577.91

Authors

Liu, J.,Deng, P. (deposition date: 2021-08-16, release date: 2022-08-17, Last modification date: 2023-03-01)

• Primary citation: Yang, M.,Sun, R.,Deng, P.,Yang, Y.,Wang, W.,Liu, J.G.,Chen, C.; Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search.; Chem Sci, 12:12776-12784, 2021; PubMed: 34703564; DOI: 10.1039/d1sc02633j

Experimental method

ELECTRON MICROSCOPY (5.26 Å)