7V59

Cryo-EM structure of spyCas9-sgRNA-DNA dimer

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004520	molecular_function	DNA endonuclease activity
B	0004527	molecular_function	exonuclease activity
B	0008408	molecular_function	3'-5' exonuclease activity
B	0043571	biological_process	maintenance of CRISPR repeat elements
B	0046872	molecular_function	metal ion binding
B	0051607	biological_process	defense response to virus
E	0003676	molecular_function	nucleic acid binding
E	0003677	molecular_function	DNA binding
E	0003723	molecular_function	RNA binding
E	0004519	molecular_function	endonuclease activity
E	0004520	molecular_function	DNA endonuclease activity
E	0004527	molecular_function	exonuclease activity
E	0008408	molecular_function	3'-5' exonuclease activity
E	0043571	biological_process	maintenance of CRISPR repeat elements
E	0046872	molecular_function	metal ion binding
E	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
B	ASP10
E	ASP10

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
B	HIS840
E	HIS840

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site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:24505130

Chain	Residue	Details
B	ASP10
B	GLU762
B	GLU766
B	HIS983
B	HIS1297
B	ASP1328
E	ASP10
E	GLU762
E	GLU766
E	HIS983
E	HIS1297
E	ASP1328

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