7V59
Cryo-EM structure of spyCas9-sgRNA-DNA dimer
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.26 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
