7S4V
Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation
Summary for 7S4V
Entry DOI | 10.2210/pdb7s4v/pdb |
EMDB information | 24833 24834 24835 24836 24837 24838 |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, gRNA, TS, ... (4 entities in total) |
Functional Keywords | cas9, mismatch, immune system-dna-rna complex, immune system/dna/rna |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 4 |
Total formula weight | 209854.47 |
Authors | Bravo, J.P.K.,Taylor, D.W.,Liu, M.S.,Johnson, K.A. (deposition date: 2021-09-09, release date: 2022-03-02, Last modification date: 2024-06-05) |
Primary citation | Bravo, J.P.K.,Liu, M.S.,Hibshman, G.N.,Dangerfield, T.L.,Jung, K.,McCool, R.S.,Johnson, K.A.,Taylor, D.W. Structural basis for mismatch surveillance by CRISPR-Cas9. Nature, 603:343-347, 2022 Cited by PubMed: 35236982DOI: 10.1038/s41586-022-04470-1 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.28 Å) |
Structure validation
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