7S3H

Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation

Summary for 7S3H

Entry DOI	10.2210/pdb7s3h/pdb
Related	7S36 7S37 7S38
EMDB information	24817 24818 24819 24823
Descriptor	Non-target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, Single-guide RNA, ... (4 entities in total)
Functional Keywords	dnase, complex, ribonucleoprotein, genome editor, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	4
Total formula weight	210266.52
Authors	Cofsky, J.C.,Soczek, K.M.,Knott, G.J.,Nogales, E.,Doudna, J.A. (deposition date: 2021-09-06, release date: 2022-04-20, Last modification date: 2022-05-04)
Primary citation	Cofsky, J.C.,Soczek, K.M.,Knott, G.J.,Nogales, E.,Doudna, J.A. CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29:395-402, 2022 Cited by PubMed: 35422516 DOI: 10.1038/s41594-022-00756-0 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.5 Å)

Structure validation