4OO8

Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA

Summary for 4OO8

• Entry DOI: 10.2210/pdb4oo8/pdb
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, RNA (97-MER), DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3'), ... (4 entities in total)
• Functional Keywords: hydrolase-dna-rna complex, hydrolase/dna/rna
• Biological source: Streptococcus pyogenes serotype M1
• Total number of polymer chains: 6
• Total formula weight: 395168.71

Authors

Nishimasu, H.,Ishitani, R.,Nureki, O. (deposition date: 2014-01-31, release date: 2014-02-26, Last modification date: 2024-04-03)

• Primary citation: Nishimasu, H.,Ran, F.A.,Hsu, P.D.,Konermann, S.,Shehata, S.I.,Dohmae, N.,Ishitani, R.,Zhang, F.,Nureki, O.; Crystal structure of Cas9 in complex with guide RNA and target DNA; Cell(Cambridge,Mass.), 156:935-949, 2014; PubMed: 24529477; DOI: 10.1016/j.cell.2014.02.001

Experimental method

X-RAY DIFFRACTION (2.5 Å)