4OO8
Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1372 | 158964.1 | 2 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | |
| 2 | B, E | RNA (97-MER) | polymer | 98 | 31689.8 | 2 | |||
| 3 | C, F | DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3') | polymer | 23 | 6930.5 | 2 | |||
| 4 | water | water | 18.0 | 144 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Leu 80 | Cys 80 | engineered mutation |
| Glu 574 | Cys 574 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 395168.7 | |
| All* | Total formula weight | 395168.7 |
