6K3Z
Crystal structure of dCas9 in complex with sgRNA and DNA (TGA PAM)
Summary for 6K3Z
Entry DOI | 10.2210/pdb6k3z/pdb |
Descriptor | RNA (81-MER), CRISPR-associated endonuclease Cas9, DNA (28-MER), ... (4 entities in total) |
Functional Keywords | endonuclease, hydrolase-dna-rna complex, hydrolase/dna/rna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 4 |
Total formula weight | 197654.80 |
Authors | |
Primary citation | Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q. Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. Plos Biol., 17:e3000496-e3000496, 2019 Cited by PubMed: 31603896DOI: 10.1371/journal.pbio.3000496 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
Download full validation report