8PM4
Cryo-EM structure of the Cas12m-crRNA-target DNA complex
Summary for 8PM4
| Entry DOI | 10.2210/pdb8pm4/pdb |
| EMDB information | 17757 |
| Descriptor | Transposase, crRNA, chain B, DNA oligoduplex, target strand, chain C, ... (4 entities in total) |
| Functional Keywords | cas12, cas12m, crispr-cas, ruvc, crrna, pam, rna binding protein |
| Biological source | Gordonia otitidis NBRC 100426 More |
| Total number of polymer chains | 4 |
| Total formula weight | 114075.30 |
| Authors | Sasnauskas, G.,Tamulaitiene, G.,Karvelis, T.,Bigelyte, G.,Siksnys, V. (deposition date: 2023-06-28, release date: 2024-02-07, Last modification date: 2024-04-24) |
| Primary citation | Bigelyte, G.,Duchovska, B.,Zedaveinyte, R.,Sasnauskas, G.,Sinkunas, T.,Dalgediene, I.,Tamulaitiene, G.,Silanskas, A.,Kazlauskas, D.,Valancauskas, L.,Madariaga-Marcos, J.,Seidel, R.,Siksnys, V.,Karvelis, T. Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing. Nucleic Acids Res., 52:3234-3248, 2024 Cited by PubMed: 38261981DOI: 10.1093/nar/gkae016 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.93 Å) |
Structure validation
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