8PM4
Cryo-EM structure of the Cas12m-crRNA-target DNA complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.93 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX,Coot |
| Symmetry | C1 |
| CTF correction | NONE |
