8PM4
Cryo-EM structure of the Cas12m-crRNA-target DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Transposase | polymer | 614 | 68223.6 | 1 | UniProt (H5TRP0) | Gordonia otitidis NBRC 100426 | Cas12m protein |
| 2 | B (B) | crRNA, chain B | polymer | 58 | 18751.2 | 1 | Gordonia otitidis NBRC 100426 | ||
| 3 | C (C) | DNA oligoduplex, target strand, chain C | polymer | 44 | 13556.7 | 1 | synthetic construct | ||
| 4 | D (D) | DNA oligoduplex, non-target strand, chain D | polymer | 44 | 13543.7 | 1 | synthetic construct |
Sequence modifications
A: 1 - 607 (UniProt: H5TRP0)
| PDB | External Database | Details |
|---|---|---|
| Ser -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 114075.3 | |
| All* | Total formula weight | 114075.3 |
