5F9R

Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage

Summary for 5F9R

Entry DOI	10.2210/pdb5f9r/pdb
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, RNA (116-MER), DNA (30-MER), ... (5 entities in total)
Functional Keywords	crispr, cas9, r-loop, genome engineering, hydrolase-dna-rna complex, hydrolase/dna/rna
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	4
Total formula weight	215728.56
Authors	Jiang, F.,Doudna, J.A. (deposition date: 2015-12-10, release date: 2016-01-27, Last modification date: 2024-03-06)
Primary citation	Jiang, F.,Taylor, D.W.,Chen, J.S.,Kornfeld, J.E.,Zhou, K.,Thompson, A.J.,Nogales, E.,Doudna, J.A. Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Science, 351:867-871, 2016 Cited by PubMed: 26841432 DOI: 10.1126/science.aad8282 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.4 Å)

Structure validation