5F9R
Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116,0.98,0.957 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 147.940, 230.100, 417.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.725 - 3.400 |
| R-factor | 0.2399 |
| Rwork | 0.237 |
| R-free | 0.29860 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.481 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.000 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.520 | |
| Number of reflections | 48447 | |
| <I/σ(I)> | 6.7 | 1.9 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.8 M ammonium sulfate, 100 mM Tris-HCl pH8.0 and 10 mM EDTA |
