5F9R
Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158699.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpyCas9 |
| 2 | B (A) | RNA (116-MER) | polymer | 118 | 38292.6 | 1 | Streptococcus pyogenes MGAS8232 | ||
| 3 | C (C) | DNA (30-MER) | polymer | 30 | 9085.8 | 1 | Lambdapapillomavirus 1 | ||
| 4 | D (D) | DNA (5'-D(P*AP*TP*GP*AP*GP*AP*CP*GP*CP*TP*GP*GP*AP*GP*TP*AP*CP*AP*C)-3') | polymer | 30 | 9362.0 | 1 | Lambdapapillomavirus 1 | ||
| 5 | E, F, G (B, A, C) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 215440.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 288.2 | |
| All* | Total formula weight | 215728.6 |
